Gu Rui, Wan Zhi Qiang, Tang Fang, Liu Xue Ting, Yang Yan Ting, Shi Feng Ling
Key Laboratory of Grassland Resources of Ministry of Education, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
College of Geographical Science, Inner Mongolia Normal University, Hohhot, China.
Front Plant Sci. 2023 Aug 29;14:1173191. doi: 10.3389/fpls.2023.1173191. eCollection 2023.
Land salinization considerably limits crop production. Biological improvement of saline and alkaline land is an important way to achieve efficient land use. It is crucial to study the salt tolerance of halophyte resources in order to explore and improve plant resources through biological improvement. is a mesophyte halophyte with strong salt tolerance. In this study, we conducted research on the salt tolerance mechanism of through phenotypic, physiological, and transcriptomic aspects. The results indicate that leaf cross-sections revealed that has a salt gland tissue composed of stalk, collecting, and secretory cells, which are trapped in epidermal cells. At the physiological level, the maximum salt tolerance threshold of leaves was 600 mM/L. At this concentration, proline content, relative conductivity, and superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) enzyme activities were maximum. At the transcriptional level, transcriptome data of three experimental groups (N0: 0 mM/L, N3: 600 mM/L, and N4: 800 mM/L) were analyzed, and six essential genes related to proline synthesis and five essential genes related to SOD and CAT enzyme activities were identified. Two genes involved in CAT enzyme activity were also found to play an important role in the MAPK signaling pathway. Trend analysis revealed that the MAPK signaling regulation (37 differentially expressed genes (DEGs)), phytohormone regulation (48 DEGs), glutathione metabolism (8 DEGs), flavonoid and flavonoid biosynthesis (2DEGs), and flavonoid biosynthesis (24 DEGs) pathways played important roles in regulating the salt tolerance of . These findings provide valuable information for further studies on the functional characteristics of in response to abiotic stress and may contribute to salt resistance breeding of fodder crops for cultivation in saline alkali land.
土地盐碱化严重限制了作物产量。盐碱地的生物改良是实现土地高效利用的重要途径。研究盐生植物资源的耐盐性对于通过生物改良探索和改良植物资源至关重要。[植物名称]是一种具有较强耐盐性的中生盐生植物。在本研究中，我们从表型、生理和转录组学方面对[植物名称]的耐盐机制进行了研究。结果表明，叶片横切面显示[植物名称]具有由柄细胞、收集细胞和分泌细胞组成的盐腺组织，这些细胞被困在表皮细胞中。在生理水平上，[植物名称]叶片的最大耐盐阈值为600 mM/L。在此浓度下，脯氨酸含量、相对电导率以及超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的酶活性均达到最大值。在转录水平上，对三个实验组（N0：0 mM/L、N3：600 mM/L和N4：800 mM/L）的转录组数据进行了分析，鉴定出六个与脯氨酸合成相关的关键基因以及五个与SOD和CAT酶活性相关的关键基因。还发现两个参与CAT酶活性的基因在MAPK信号通路中发挥重要作用。趋势分析表明，MAPK信号调控（37个差异表达基因（DEGs））、植物激素调控（48个DEGs）、谷胱甘肽代谢（8个DEGs）、黄酮类和黄酮生物合成（2个DEGs）以及黄酮生物合成（24个DEGs）途径在调节[植物名称]的耐盐性方面发挥重要作用。这些发现为进一步研究[植物名称]响应非生物胁迫的功能特性提供了有价值的信息，并可能有助于盐碱地种植的饲料作物抗盐育种。
