Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece.
Bruker Daltonics GmbH, Fahrenheitstr. 4, 28359 Bremen, Germany.
Food Chem. 2024 Feb 15;434:137410. doi: 10.1016/j.foodchem.2023.137410. Epub 2023 Sep 13.
Metabolomics is widely established in the field of food authenticity to address demanding issues, such as adulteration cases. Trapped ion mobility spectrometry (TIMS) coupled to liquid chromatography (LC) and high-resolution mass spectrometry (HRMS) provides an additional analytical dimension, introducing mobility-enhanced metabolomics in four dimensions (4D). In the present work, the potential of LC-TIMS-HRMS as a reliable analytical platform for authenticity studies is being explored, applied in extra virgin olive oil (EVOO) adulteration study. An integrated untargeted 4D-metabolomics approach is being implemented to investigate adulteration, with refined olive oils (ROOs) and olive pomace oils (OPOs) set as adulterants. Robust prediction models are built, successfully discriminating authentic EVOOs from adulterated ones and highlighting markers in each group. Noteworthy outcomes are retrieved regarding TIMS added value in LC-HRMS workflows, resulting in a significant increase of metabolic coverage, while, thanks to platform's enhanced sensitivity, detection of adulteration is being achieved down to 1%.
代谢组学在食品真实性领域得到了广泛应用，以解决掺假等棘手问题。离子淌度谱（TIMS）与液相色谱（LC）和高分辨率质谱（HRMS）相结合，提供了额外的分析维度，在四维（4D）中引入了增强迁移率的代谢组学。本研究探讨了 LC-TIMS-HRMS 作为真实性研究可靠分析平台的潜力，并将其应用于特级初榨橄榄油（EVOO）掺假研究。采用集成的非靶向 4D 代谢组学方法研究掺假，以精制橄榄油（ROO）和橄榄渣油（OPO）作为掺杂物。建立了稳健的预测模型，成功地区分了真伪 EVOO，并突出了每组的标志物。值得注意的是，TIMS 在 LC-HRMS 工作流程中的附加值，显著增加了代谢物的覆盖范围，同时，由于平台的增强灵敏度，在 1%的水平下即可检测到掺假。
