Behr Jan Helge, Kampouris Ioannis D, Babin Doreen, Sommermann Loreen, Francioli Davide, Kuhl-Nagel Theresa, Chowdhury Soumitra Paul, Geistlinger Joerg, Smalla Kornelia, Neumann Günter, Grosch Rita
Leibniz Institute of Vegetable and Ornamental Crops (IGZ), Plant-Microbe Systems, Großbeeren, Germany.
Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Braunschweig, Germany.
Front Plant Sci. 2023 Aug 28;14:1232288. doi: 10.3389/fpls.2023.1232288. eCollection 2023.
The beneficial effect of microbial consortium application on plants is strongly affected by soil conditions, which are influenced by farming practices. The establishment of microbial inoculants in the rhizosphere is a prerequisite for successful plant-microorganism interactions. This study investigated whether a consortium of beneficial microorganisms establishes in the rhizosphere of a winter crop during the vegetation period, including the winter growing season. In addition, we aimed for a better understanding of its effect on plant performance under different farming practices. Winter rye plants grown in a long-time field trial under conventional or organic farming practices were inoculated after plant emergence in autumn with a microbial consortium containing sp. (RU47), (ABi03) and (OMG16). The density of the microbial inoculants in the rhizosphere and root-associated soil was quantified in autumn and the following spring. Furthermore, the influence of the consortium on plant performance and on the rhizosphere bacterial community assembly was investigated using a multidisciplinary approach. Selective plating showed a high colonization density of individual microorganisms of the consortium in the rhizosphere and root-associated soil of winter rye throughout its early growth cycle. 16S rRNA gene amplicon sequencing showed that the farming practice affected mainly the rhizosphere bacterial communities in autumn and spring. However, the microbial consortium inoculated altered also the bacterial community composition at each sampling time point, especially at the beginning of the new growing season in spring. Inoculation of winter rye with the microbial consortium significantly improved the plant nutrient status and performance especially under organic farming. In summary, the microbial consortium showed sufficient efficacy throughout vegetation dormancy when inoculated in autumn and contributed to better plant performance, indicating the potential of microbe-based solutions in organic farming where nutrient availability is limited.
微生物群落应用对植物的有益影响受到土壤条件的强烈影响，而土壤条件又受耕作方式的影响。微生物接种剂在根际的定殖是植物与微生物成功相互作用的先决条件。本研究调查了有益微生物群落在包括冬季生长季节在内的植被期是否能在冬季作物的根际定殖。此外，我们旨在更好地了解其在不同耕作方式下对植物性能的影响。在传统或有机耕作方式下进行长期田间试验种植的冬黑麦植株，于秋季出苗后接种含有sp.（RU47）、（ABi03）和（OMG16）的微生物群落。在秋季和次年春季对根际和根际相关土壤中微生物接种剂的密度进行了定量。此外，采用多学科方法研究了该群落对植物性能和根际细菌群落组装的影响。选择性平板培养显示，在冬黑麦的整个早期生长周期中，该群落的单个微生物在根际和根际相关土壤中具有较高的定殖密度。16S rRNA基因扩增子测序表明，耕作方式主要影响秋季和春季的根际细菌群落。然而，接种的微生物群落也改变了每个采样时间点的细菌群落组成，尤其是在春季新生长季节开始时。用微生物群落接种冬黑麦显著改善了植物的养分状况和性能，尤其是在有机耕作方式下。总之，秋季接种微生物群落时，其在整个植被休眠期都显示出足够的功效，并有助于植物表现更好，这表明在养分有效性有限的有机耕作中，基于微生物的解决方案具有潜力。
