Hafiz Fatema Binte, Moradtalab Narges, Goertz Simon, Rietz Steffen, Dietel Kristin, Rozhon Wilfried, Humbeck Klaus, Geistlinger Joerg, Neumann Günter, Schellenberg Ingo
Department of Agriculture, Ecotrophology, and Landscape Development, Anhalt University of Applied Sciences, 06406 Bernburg, Saxony-Anhalt, Germany.
Institute of Crop Sciences, University of Hohenheim, 70593 Stuttgart, Baden-Württemberg, Germany.
Mol Plant Microbe Interact. 2022 May;35(5):380-392. doi: 10.1094/MPMI-11-21-0274-R. Epub 2022 Mar 23.
Rhizosphere-competent microbes often interact with plant roots and exhibit beneficial effects on plant performance. Numerous bacterial and fungal isolates are able to prime host plants for fast adaptive responses against pathogen attacks. Combined action of fungi and bacteria may lead to synergisms exceeding effects of single strains. Individual beneficial fungi and bacteria have been extensively studied in , but little is known about their concerted actions in the Brassicaceae. Here, an in-vitro system with oilseed rape () was established. Roots of two different cultivars were inoculated with well-characterized fungal ( OMG16) and bacterial ( FZB42) isolates alone or in combination. Microscopic analysis confirmed that OMG16 hyphae entered root hairs through root hair tips and formed distinct intracellular structures. Quantitative PCR revealed that root colonization of OMG16 increased up to 10-fold in the presence of FZB42. Relative transcript levels of the ethylene- and jasmonic acid-responsive genes , , and were recorded in leaves by quantitative reverse transcription PCR to measure induced systemic resistance in tissues distant from the roots. Combined action of OMG16 and FZB42 induced transcript abundances more efficiently than single inoculation. Importantly, microbial priming reduced root infection in rapeseed by approximately 100-fold compared with nonprimed plants. Priming also led to faster and stronger systemic responses of the defense genes , , , and .[Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
根际微生物通常与植物根系相互作用，并对植物生长表现出有益影响。许多细菌和真菌分离株能够使宿主植物对病原体攻击产生快速适应性反应。真菌和细菌的联合作用可能会产生超过单一菌株效果的协同作用。单个有益真菌和细菌已得到广泛研究，但它们在十字花科植物中的协同作用却知之甚少。在此，建立了一个油菜体外系统。将两种不同品种的根系单独或联合接种特征明确的真菌（OMG16）和细菌（FZB42）分离株。显微镜分析证实，OMG16菌丝通过根毛尖端进入根毛，并形成独特的细胞内结构。定量PCR显示，在FZB42存在的情况下，OMG16的根定殖增加了10倍。通过定量逆转录PCR记录叶片中乙烯和茉莉酸反应基因、和的相对转录水平，以测量根系远端组织中的诱导系统抗性。OMG16和FZB42的联合作用比单一接种更有效地诱导转录丰度。重要的是，与未引发的植物相比，微生物引发使油菜根感染减少了约100倍。引发还导致防御基因、、、的系统反应更快、更强。[公式：见正文] 版权所有© 2022作者。本文是一篇根据CC BY-NC-ND 4.0国际许可发布的开放获取文章。
