Jiao Weiting, Zhu Lei, Shen Tingting, Wang Luyao, Li Qing X, Wang Chen, Wu Xiangwei, Chen Hongping, Hua Rimao
School of Resource & Environment of Anhui Agricultural University, Key Laboratory of Agri-food Safety of Anhui Province, Hefei 230036, China; State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, China.
School of Resource & Environment of Anhui Agricultural University, Key Laboratory of Agri-food Safety of Anhui Province, Hefei 230036, China; Tea Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tea Quality and Safety & Risk Assessment, Ministry of Agriculture, Hangzhou 310008, China.
Food Chem. 2024 Feb 15;434:137305. doi: 10.1016/j.foodchem.2023.137305. Epub 2023 Aug 26.
Pyrrolizidine alkaloids (PAs) and their N-oxides (PANOs) are novel contaminants in tea. However, the source of PA/PANO contamination in tea remains unclear. In this study, 15 PAs/PANOs were extracted from plant samples (tea, fresh tea leaves, and weeds) with 0.1 M sulfuric acid and from soil with 0.1 M sulfuric acid methanol after adjusting soil acidity with 0.1 M trisodium citrate. Satisfactory recoveries of PAs/PANOs from four different matrices at 0.02, 0.1, and 0.5 mg kg was 72%-114% with relative standard deviations (RSD) of 0.03%-16%. Seven out of 15 PAs/PANOs were detected in tea purchased from the local market ranging from undetected to 96.2 μg kg. Thirteen, three, and four PAs/PANOs were detected in weeds, fresh tea leaves, and soil, respectively. Based on the types of PAs/PANOs detected in the three matrices, it was preliminarily speculated that PAs/PANOs in tea originated from weeds in the tea garden.
吡咯里西啶生物碱（PAs）及其氮氧化物（PANOs）是茶叶中的新型污染物。然而，茶叶中PA/PANO污染的来源仍不清楚。在本研究中，用0.1M硫酸从植物样品（茶叶、鲜茶叶和杂草）中提取15种PA/PANOs，在用0.1M柠檬酸三钠调节土壤酸度后，用0.1M硫酸甲醇从土壤中提取。在0.02、0.1和0.5mg/kg的添加水平下，从四种不同基质中回收PA/PANOs的回收率令人满意，为72%-114%，相对标准偏差（RSD）为0.03%-16%。在从当地市场购买的茶叶中检测到15种PA/PANOs中的7种，含量从未检测到96.2μg/kg不等。在杂草、鲜茶叶和土壤中分别检测到13种、3种和4种PA/PANOs。根据在三种基质中检测到的PA/PANOs类型，初步推测茶叶中的PA/PANOs来源于茶园中的杂草。
