Department of Chemistry, Faculty of Engineering and Technology, SRM Institute of Science and Technology, Chennai, Kattankulathur, India.
Department of Organic Chemistry, Indian Institute of Science, Bangalore, India.
Chemosphere. 2023 Nov;342:140177. doi: 10.1016/j.chemosphere.2023.140177. Epub 2023 Sep 17.
Rising concerns of pesticide-induced neurotoxicity and neurodegenerative diseases like Parkinson's, Alzheimer's, and Multiple Sclerosis, are exacerbated by overexposure to contaminated waterbodies. Therefore, evaluating the risk accurately requires reliable monitoring of related biomarkers like dopamine (DA) through electrochemical detection. Layered double hydroxides (LDHs) have shown great potential in sensors. However, to meet the challenges of rapid detection of large patient cohorts in real-time biological media, they should be further tailored to display superior analytical readouts. Herein, a ternary LDH (NiCoMn) was integrated with the sheets of thermally reduced graphene oxide (trGO), to expose more highly active edge planes of the LDH, as opposed to its generally observed inert basal planes. The improvement in detection performance through such a modulated structure-property is a prospect that hasn't been previously explored for any other LDH-based materials employed in sensing applications. The 2 folds superior electrochemical activity exhibited by the face-on oriented LDH with trGO as compared to the pristine LDH material was further employed for direct detection of DA in real blood plasma samples. Moreover, the designed sensor exhibited exceptional selectivity towards the detection of DA with a limit of detection of 34.6 nM for a wide dynamic range of 0.001-5 mM with exceptional stability retaining 88.56% of the initial current even after storage in ambient conditions for 30 days.
由于过度暴露于受污染的水体，人们对农药诱导的神经毒性和神经退行性疾病（如帕金森病、阿尔茨海默病和多发性硬化症）的担忧日益加剧。因此，要准确评估风险，需要通过电化学检测可靠地监测相关生物标志物，如多巴胺（DA）。层状双氢氧化物（LDH）在传感器方面具有巨大的潜力。然而，为了满足实时生物介质中大患者群体快速检测的挑战，它们应该进一步进行定制，以显示出更优越的分析读出能力。在此，将三元 LDH（NiCoMn）与热还原氧化石墨烯（trGO）的片层集成在一起，以暴露 LDH 的更多高活性边缘平面，而不是通常观察到的惰性基面。通过这种调制的结构-性能提高检测性能的前景，在以前的任何其他用于传感应用的基于 LDH 的材料中都没有被探索过。与原始 LDH 材料相比，具有 trGO 的面内取向 LDH 的电化学活性提高了 2 倍，可用于直接检测真实血浆样品中的 DA。此外，所设计的传感器对 DA 的检测表现出出色的选择性，具有 34.6 nM 的检测限和 0.001-5 mM 的宽动态范围，在环境条件下储存 30 天后，其初始电流仍保持 88.56%。
