Wang Yusheng, Bai Yunlong, Zeng Qi, Jiang Zeyuan, Liu Yuzhe, Wang Xiyan, Liu Xiaoting, Liu Chunlei, Min Weihong
College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China.
College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 3):126916. doi: 10.1016/j.ijbiomac.2023.126916. Epub 2023 Sep 15.
L-aspartic acid, L-threonine, L-isoleucine, l-lysine, and L-methionine constitute the l-aspartate amino acids (AFAAs). Except for L-aspartic acid, these are essential amino acids that cannot be synthesized by humans or animals themselves. E. coli and C. glutamicum are the main model organisms for AFAA production. It is necessary to reconstitute microbial cell factories and the physiological state of industrial fermentation cells for in-depth research into strains with higher AFAA production levels and optimal growth states. Considering that the anabolic pathways of the AFAAs and engineering modifications have rarely been reviewed in the latest progress, this work reviews the central metabolic pathways of two strains and strategies for the metabolic engineering of AFAA synthetic pathways. The challenges posed by microbial physiology in AFAA production and possible strategies to address them, as well as future research directions for constructing strains with high AFAA production levels, are discussed in this review article.
L-天冬氨酸、L-苏氨酸、L-异亮氨酸、L-赖氨酸和L-甲硫氨酸构成了L-天冬氨酸类氨基酸（AFAAs）。除L-天冬氨酸外，这些都是人体或动物自身无法合成的必需氨基酸。大肠杆菌和谷氨酸棒杆菌是生产AFAAs的主要模式生物。有必要重建微生物细胞工厂以及工业发酵细胞的生理状态，以便深入研究具有更高AFAAs生产水平和最佳生长状态的菌株。鉴于最新进展中很少对AFAAs的合成途径和工程改造进行综述，本文综述了这两种菌株的中心代谢途径以及AFAAs合成途径的代谢工程策略。本文还讨论了微生物生理学在AFAAs生产中带来的挑战以及应对这些挑战的可能策略，以及构建高AFAAs生产水平菌株的未来研究方向。
