Key Laboratory of Applied Surface and Colloid Chemistry Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710119, Shaanxi Province, P. R. China.
Chem Commun (Camb). 2023 Oct 4;59(79):11851-11854. doi: 10.1039/d3cc03788f.
A rapid lateral flow assay (LFA) is developed for the colorimetric and surface-enhanced Raman scattering (SERS) dual-mode detection of microRNA (miRNA) based on the robust immunoassay-like (immuno-like) recognition mechanism of S9.6 antibody to DNA/miRNA duplexes. Different from the traditional target-mediated sandwich-type hybridization-based LFA methods, the formation of S9.6 antibody/miRNA/DNA complexes is more rapid and stable, achieving 40 times higher sensitivity with only 10 min assaying time. Furthermore, taking benefit of the versatility of the immuno-like recognition mode, the multiplexed detection of miRNAs can be realized with the SERS signal readout, providing a versatile LFA design towards sensitive, specific, and multiplexed miRNA analysis.
一种基于 S9.6 抗体对 DNA/miRNA 双链体的稳健免疫类似（immuno-like）识别机制的比色和表面增强拉曼散射（SERS）双模检测微 RNA（miRNA）的快速侧向流动分析（LFA）被开发出来。与传统的基于靶介导的夹心型杂交的 LFA 方法不同，S9.6 抗体/miRNA/DNA 复合物的形成更快更稳定，仅需 10 分钟的测定时间，灵敏度提高了 40 倍。此外，利用免疫类似识别模式的多功能性，可以通过 SERS 信号读出实现 miRNA 的多重检测，为敏感、特异和多重 miRNA 分析提供了多功能的 LFA 设计。
