Copy number variations of cytochrome genes in Kinh Vietnamese.

BACKGROUND

The cytochrome P450 () family is well known as a major group of drug metabolizing enzymes. The polymorphism of genes is the main factor having an impact on the interindividual difference in drug response, including drug efficacy and drug safety. The single nucleotide polymorphism (SNPs) of Vietnamese Kinh has been widely studied, but information about the copy number variations (CNVs) of other genes is still unknown.

OBJECTIVE

To identify the CNV variability of in 154 healthy unrelated Kinh Vietnamese, except e, which was previously reported.

METHODS

Multiplex Ligation-Dependent Probe Amplification (MLPA) was applied for determination of copy number of 10 genes. Later, PCR or quantitative PCR (qPCR) was used to confirm the detected CNVs in randomly chosen subjects.

RESULTS

Of the 154 subjects, along with , 4 other genes showed CNVs including duplications (), deletions ( and ), and both duplications and deletions (). Among these, exhibited the greatest frequency of CNVs compared with other , in which Del accounted for 11%. Meanwhile, allele Del showed the lowest frequency with only 0.3%.

CONCLUSIONS

The present study provides new insight into CNVs in the Kinh Vietnamese cohort. Our data have contributed to genetic profiling of CNVs in Vietnam, which would be helpful for facilitating implementation of pharmacogenetics in drug dosing adjustment in Vietnam.