Liu Shaomin, Wu Jiating, Hu Ziyan, Jiang Mengyu
School of Earth and Environment, Anhui University of Science and Technology, Huainan, China.
State Key Laboratory of Mining Response and Disaster Prevention and Control in Deep Coal Mines (Anhui University of Science and Technology), Huainan, China.
Front Microbiol. 2023 Sep 1;14:1239218. doi: 10.3389/fmicb.2023.1239218. eCollection 2023.
In this study, the effects of different enzymes (lysozyme, α-amylase and neutral protease) on sludge hydrolysis efficiency and microbial community in sequencing batch reactor (SBR) were introduced. The results showed that the hydrolysis efficiencies of the three enzymes were 48.5, 22.5 and 31%, respectively, compared with the accumulated sludge discharge of the blank control group. However, it has varying degrees of impact on the effluent quality, and the denitrification and phosphorus removal effect of the system deteriorates. The lysozyme that achieves the optimal sludge hydrolysis effect of 48.5% has the greatest impact on the chemical oxygen demand (COD), total nitrogen (TN), and nitrate nitrogen (NO-N) of the effluent. The sludge samples of the control group and the groups supplemented with different enzyme preparations were subjected to high-throughput sequencing. It was found that the number of OTUs (Operational Taxonomic Units) of the samples was lysozyme > α-amylase > blank control > neutral protease. Moreover, the abundance grade curve of the sludge samples supplemented with lysozyme and α-amylase was smoother, and the community richness and diversity were improved by lysozyme and α-amylase. The species diversity of the sludge supplemented with lysozyme and neutral protease was great, and the community succession was obvious. The introduction of enzymes did not change the main microbial communities of the sludge, which were mainly Proteobacteria, Actinobacteria and Bacteroidetes. The effects of three enzyme preparations on sludge reduction and microbial diversity during pilot operation were analyzed, the gap in microbial research was filled, which provided theoretical value for the practical operation of enzymatic sludge reduction.
本研究介绍了不同酶（溶菌酶、α-淀粉酶和中性蛋白酶）对序批式反应器（SBR）中污泥水解效率和微生物群落的影响。结果表明，与空白对照组的累积污泥排放量相比，三种酶的水解效率分别为48.5%、22.5%和31%。然而，其对出水水质有不同程度的影响，系统的脱氮除磷效果变差。实现48.5%最佳污泥水解效果的溶菌酶对出水的化学需氧量（COD）、总氮（TN）和硝酸盐氮（NO-N）影响最大。对对照组和添加不同酶制剂组的污泥样品进行高通量测序。发现样品的OTU（操作分类单元）数量为溶菌酶>α-淀粉酶>空白对照>中性蛋白酶。此外，添加溶菌酶和α-淀粉酶的污泥样品的丰度等级曲线更平滑，溶菌酶和α-淀粉酶提高了群落丰富度和多样性。添加溶菌酶和中性蛋白酶的污泥物种多样性大，群落演替明显。酶的引入未改变污泥的主要微生物群落，主要为变形菌门、放线菌门和拟杆菌门。分析了三种酶制剂在中试运行期间对污泥减量和微生物多样性的影响，填补了微生物研究的空白，为酶促污泥减量的实际运行提供了理论价值。
