Mischke S, Saunders J A, Owens L
J Biochem Biophys Methods. 1986 Sep;13(2):65-75. doi: 10.1016/0165-022x(86)90018-7.
Instrumentation is described which will generate AC fields and DC square pulses for cell electrofusion, electroporation and other electrophysiological experiments. The equipment can generate a constant or intermittent AC field and a single square pulse, either alone or in combination. It can also generate a train of intermittent square pulses. The components for the instrumentation are commercially available and can be assembled by persons with little or no training in electronics. A design is given for a mass treatment chamber that can be easily constructed and viewed with a microscope.
本文介绍了一种仪器，它能产生用于细胞电融合、电穿孔及其他电生理实验的交流电场和直流方波脉冲。该设备可单独或组合产生恒定或间歇的交流电场以及单个方波脉冲。它还能产生一系列间歇方波脉冲。该仪器的组件可从市场购得，几乎没有或完全没有电子学培训的人员也能进行组装。文中给出了一种易于构建且可通过显微镜观察的大规模处理室的设计方案。
