Department of Animal Sciences, Donald Henry Barron Reproductive and Perinatal Biology Research Program, and the Genetics Institute, University of Florida, Gainesville, Florida, United States.
School of Agriculture and Food Science, University College Dublin, Dublin, Ireland.
Physiol Genomics. 2023 Nov 1;55(11):557-564. doi: 10.1152/physiolgenomics.00064.2023. Epub 2023 Sep 18.
The objectives of the present study were to characterize the expression of genes encoding for cell signaling ligands in the bovine endosalpinx and endometrium and analyze spatial changes in gene expression. RNA sequencing was performed for the endosalpinx from the ampulla of the oviduct and endometrium from the upper and middle uterine horn and uterine body at after ovulation from ipsilateral and contralateral sides relative to the ovulatory ovary. Of the 17,827 unique mRNA transcripts mapped, 2,072 were affected by cranial-caudal position in the reproductive tract and 818 were affected by side (false discovery rate < 0.05). There were 334 genes encoding for cell signaling ligands, with 128 genes having greater than two transcripts per million on average. A total of 81 cell signaling ligand genes were affected by position and 24 were affected by side. A data set of the transcriptome of two to four cell embryos was used to identify cell signaling ligand genes that were highly expressed in the ampulla for which there was high expression of the receptor in the embryo. The most expressed ligand-receptor pairs were , , , and . These cell signaling ligands, as well as others whose gene is expressed in the endosalpinx and endometrium, may influence early embryonic development. Spatial changes throughout the reproductive tract highlight the distinctive expression profile of the oviduct versus the endometrium, including a set of the identified genes encoding for cell signaling ligands, and highlight the local influence of the ovary. The results also show the continuity of expression for large numbers of genes in the reproductive tract. Examination of the transcriptome of the endosalpinx and endometrium revealed the degree to which gene expression in the reproductive tract varies spatially. The expression of genes encoding cell signaling molecules that could potentially regulate embryonic development was also identified.
本研究的目的是描述牛输卵管内囊和子宫内膜中细胞信号配体编码基因的表达，并分析基因表达的空间变化。从排卵后同侧和对侧相对于排卵卵巢的输卵管内囊和子宫角上部和中部及子宫体进行 RNA 测序。在 17827 个独特的 mRNA 转录本中，有 2072 个受生殖道头-尾位置影响，818 个受侧位影响（错误发现率<0.05）。有 334 个基因编码细胞信号配体，其中 128 个基因的平均转录本数大于 2 个每百万。共有 81 个细胞信号配体基因受位置影响，24 个受侧位影响。使用 2 到 4 个细胞胚胎的转录组数据集来鉴定在胚胎中高表达受体的内囊中高度表达的细胞信号配体基因。表达最丰富的配体-受体对为、、、和。这些细胞信号配体，以及其他在内囊中表达的基因，可能影响早期胚胎发育。生殖道内的空间变化突出了输卵管与子宫内膜的独特表达模式，包括一组鉴定出的编码细胞信号配体的基因，并强调了卵巢的局部影响。结果还显示了生殖道内大量基因表达的连续性。对内囊和子宫内膜的转录组进行检查，揭示了生殖道基因表达在空间上的变化程度。还鉴定了可能调节胚胎发育的细胞信号分子编码基因的表达。
