Vroman L, Adams A L
J Immunol Methods. 1986 Nov 6;93(2):213-6. doi: 10.1016/0022-1759(86)90191-2.
Suspensions of Fe3O4 (black), Fe2O3 (red), and Cr2O3 (green) were exposed to solutions of protein A, and then each to a different antiserum to one of the following human proteins: fibrinogen, high molecular weight kininogen (HMK), albumin or immunoglobulins (IgG). Test surfaces were patterns of human proteins adsorbed out of solutions or out of plasma, onto glass as well as onto polyvinylchloride slides. They were exposed to single or mixed suspensions of the treated oxides for about 30 s and rinsed. Adhesion of each oxide onto each matching protein of these patterned test surfaces resulted, thus identifying each protein by color.
将四氧化三铁(黑色)、三氧化二铁(红色)和三氧化二铬(绿色)的悬浮液暴露于蛋白A溶液中,然后分别将每种悬浮液与针对以下人类蛋白质之一的不同抗血清接触:纤维蛋白原、高分子量激肽原(HMK)、白蛋白或免疫球蛋白(IgG)。测试表面是从溶液或血浆中吸附到玻璃以及聚氯乙烯载玻片上的人类蛋白质图案。将它们暴露于经处理的氧化物的单一或混合悬浮液中约30秒,然后冲洗。由此产生了每种氧化物在这些有图案的测试表面上与每种匹配蛋白质的粘附,从而通过颜色识别每种蛋白质。