酪氨酰-5'-腺苷酸与酪氨酰-tRNA合成酶（大肠杆菌）的结合。
The binding of tyrosinyl-5'-AMP to tyrosyl-tRNA synthetase (E.coli).
作者信息
Grosse F, Krauss G, Kownatzki R, Maass G
出版信息
Nucleic Acids Res. 1979 Apr;6(4):1631-8. doi: 10.1093/nar/6.4.1631.
Abstract
The binding between tyrosyl-tRNA synthetase (E.coli) and the alkylanalogue of the aminoacyladenylate, tyrosinyl-5'-AMP, has been investigated by fluorescence titrations and rapid mixing experiments. Tyrosyl-tRNA synthetase has two equivalent and independent binding sites for tyrosinyl-5'-AMP. The intrinsic binding constant is 4 x 10(7)M-1. The binding sites for tRNATyr and tyrosinyl-5'-AMP are independent of each other, the anticooperative mode of tRNA binding being preserved in the presence of tyrosinyl-5-AMP.
摘要
通过荧光滴定和快速混合实验研究了酪氨酰 - tRNA合成酶（大肠杆菌）与氨酰腺苷酸的烷基类似物酪氨酰 - 5'-AMP之间的结合。酪氨酰 - tRNA合成酶对酪氨酰 - 5'-AMP有两个等效且独立的结合位点。内在结合常数为4×10⁷M⁻¹。tRNATyr和酪氨酰 - 5'-AMP的结合位点相互独立，在酪氨酰 - 5-AMP存在下，tRNA结合的反协同模式得以保留。
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本文引用的文献
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Biochemistry. 1966 May;5(5):1681-90. doi: 10.1021/bi00869a033.
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The mechanism of reaction of methionyl-tRNA synthetase from Escherichia coli. Interaction of the enzyme with ligands of the amino-acid-activation reaction.大肠杆菌甲硫氨酰 - tRNA合成酶的反应机制。该酶与氨基酸激活反应配体的相互作用。
Eur J Biochem. 1972 Jan 21;24(3):461-9. doi: 10.1111/j.1432-1033.1972.tb19707.x.
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The mechanism of action of methionyl-tRNA synthetase from Escherichia coli. 1. Fluorescence studies on tRNAMet binding as a function of ligands, ions and pH.来自大肠杆菌的甲硫氨酰 - tRNA合成酶的作用机制。1. 作为配体、离子和pH函数的tRNAMet结合的荧光研究。
Eur J Biochem. 1973 Jul 2;36(1):213-26. doi: 10.1111/j.1432-1033.1973.tb02903.x.
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Isoleucyl transfer ribonucleic acid synthetase of Escherichia coli B. A rapid kinetic investigation of the L-isoleucine-activating reaction.大肠杆菌B的异亮氨酰转移核糖核酸合成酶。L-异亮氨酸激活反应的快速动力学研究。
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8
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