Dai Meng, Su Yuantao, Wu Zhixiong
Department of Critical Care Medicine, Huadong Hospital, Fudan University, Shanghai, China.
Department of General Surgery, Huadong Hospital, Fudan University, Shanghai, China.
J Gene Med. 2024 Jan;26(1):e3592. doi: 10.1002/jgm.3592. Epub 2023 Sep 19.
Plakophilin 2 gene (PKP2) has been revealed to be differentially expressed in various cancer types and is correlated with prognosis. However, the role of PKP2 in colon adenocarcinoma remains indistinct.
Differences in transcriptional expression of PKP2 between colon adenocarcinoma tissues and normal adjacent tissues were acquired from the publicly available dataset-the Cancer Genome Atlas. A receiver operating curve (ROC) was constructed to differentiate colon adenocarcinoma tissues from adjacent normal tissues. The Kaplan-Meier plot method was performed to evaluate the effect of PKP2 on survival. The correlation between mRNA expression of PKP2 and immune infiltrating was determined by the Tumor Immune Estimation Resource and Tumor-Immune System Interaction databases.
The expression of PKP2 in colon adenocarcinoma tissues was significantly downregulated compared with corresponding adjacent normal tissues. Decreased PKP2 mRNA expression was associated with lymph node metastases and advanced pathological stage. The ROC curve analysis indicated that with a cutoff value of 6.034, the sensitivity and specificity for PKP2 differentiating the colon adenocarcinoma tissues from the adjacent normal tissues were 90.2 and 66.5% respectively. Kaplan-Meier plot survival analysis revealed that colon adenocarcinoma patients with low-PKP2 had a worse prognosis than those with high-PKP2 (68.2 vs. 101.4 months, p = 0.028). Correlation analysis showed that mRNA expression of PKP2 was correlative with immune infiltrates.
Downregulated PKP2 is significantly correlated with unfavorable immune infiltrating and survival in colon adenocarcinoma. This research indicates that PKP2 can be selected as a novel biomarker of potential immunotherapy targets and unfavorable prognosis in colon adenocarcinoma.
盘状球蛋白2基因（PKP2）已被发现在多种癌症类型中存在差异表达，且与预后相关。然而，PKP2在结肠腺癌中的作用仍不明确。
从公开可用的数据集——癌症基因组图谱中获取结肠腺癌组织与相邻正常组织之间PKP2转录表达的差异。构建受试者工作特征曲线（ROC）以区分结肠腺癌组织与相邻正常组织。采用Kaplan-Meier绘图法评估PKP2对生存的影响。通过肿瘤免疫估计资源和肿瘤-免疫系统相互作用数据库确定PKP2的mRNA表达与免疫浸润之间的相关性。
与相应的相邻正常组织相比，结肠腺癌组织中PKP2的表达显著下调。PKP2 mRNA表达降低与淋巴结转移和晚期病理分期相关。ROC曲线分析表明，截断值为6.034时，PKP2区分结肠腺癌组织与相邻正常组织的敏感性和特异性分别为90.2%和66.5%。Kaplan-Meier绘图生存分析显示，低PKP2的结肠腺癌患者预后比高PKP2的患者差（68.2个月对101.4个月，p = 0.028）。相关性分析表明，PKP2的mRNA表达与免疫浸润相关。
PKP2下调与结肠腺癌中不良的免疫浸润和生存显著相关。本研究表明，PKP2可作为结肠腺癌潜在免疫治疗靶点和不良预后的新型生物标志物。
