Department of Urology & Andrology, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, 550001, China.
Department of Urology, Guiyang Public Health Clinical Center, Guiyang, 550002, China.
Environ Sci Pollut Res Int. 2023 Oct;30(48):106431-106441. doi: 10.1007/s11356-023-29874-y. Epub 2023 Sep 20.
Polysaccharides from Dendrobium officinale polysaccharides (DOPs) are the main bioactive components of Dendrobium officinale, which have the functions of antioxidation and immune regulation. However, it is not clear whether DOPs have any effect on the prevention of reproductive disorders induced by oxidative stress. The purpose of this study was to explore the protective effect of DOPs on reproductive oxidative stress injury in male mice and its possible mechanism. In this study, the mouse model of reproductive injury was established by intraperitoneal injection of cyclophosphamide (CTX). The reproductive function was evaluated by relative testicular mass, sperm parameters, and sex hormone levels. The oxidative stress level of male mice with reproductive injury treated with DOPs was analyzed by the levels of 8-hydroxydeoxyguanosine (8-OHdG), malondialdehyde (MDA), and nitric oxide (NO) in sperm. The expression of follicle-stimulating hormone receptor (FSHR) mRNA, androgen-binding (ABP) mRNA, and c-kit mRNA was detected by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) to explore its mechanism. After CTX administration, the sperm density, sperm motility, normal sperm morphology, and sex hormone levels in mice were significantly lower than those in the control group (P < 0.05). At the same time, the expression of p53 protein was upregulated, and the expression of Bcl-2 protein was downregulated (P < 0.05). In addition, the expression of FSHR and ABP mRNA on Sertoli cells was also significantly inhibited (P < 0.05). DOPs can effectively reduce the oxidative stress injury of testicular tissue. After DOP treatment, the sperm quality and sex-related hormone levels of mice were significantly improved and positively correlated with the dose of DOPs (P < 0.05). Administration of DOPs can reduce the damage caused by oxidative stress by reducing the level of oxidative stress, improving the hormone environment in testes, and regulating the expression of specific genes in Sertoli cells and spermatogenic cells.
铁皮石斛多糖（DOPs）是铁皮石斛的主要生物活性成分，具有抗氧化和免疫调节功能。然而，DOPs 是否对预防氧化应激引起的生殖障碍有影响尚不清楚。本研究旨在探讨 DOPs 对雄性小鼠生殖氧化应激损伤的保护作用及其可能的机制。在这项研究中，通过腹腔注射环磷酰胺（CTX）建立了生殖损伤的小鼠模型。通过相对睾丸质量、精子参数和性激素水平来评估生殖功能。通过精子中 8-羟基脱氧鸟苷（8-OHdG）、丙二醛（MDA）和一氧化氮（NO）的水平分析接受 DOPs 治疗的生殖损伤雄性小鼠的氧化应激水平。通过定量逆转录聚合酶链反应（qRT-PCR）检测卵泡刺激素受体（FSHR）mRNA、雄激素结合蛋白（ABP）mRNA 和 c-kit mRNA 的表达，以探讨其机制。CTX 给药后，与对照组相比，小鼠的精子密度、精子活力、正常精子形态和性激素水平明显降低（P<0.05）。同时，p53 蛋白表达上调，Bcl-2 蛋白表达下调（P<0.05）。此外，Sertoli 细胞上的 FSHR 和 ABP mRNA 表达也明显受到抑制（P<0.05）。DOPs 能有效减轻睾丸组织的氧化应激损伤。经 DOP 处理后，小鼠的精子质量和性激素水平显著改善，且与 DOP 剂量呈正相关（P<0.05）。DOPs 给药可通过降低氧化应激水平、改善睾丸内激素环境以及调节 Sertoli 细胞和生精细胞中特定基因的表达，减轻氧化应激造成的损伤。
