通过低压刺激提高微生物耐盐性，以改善高盐废水中对 p-氯硝基苯（p-CNB）的去除。
Enhancing microbial salt tolerance through low-voltage stimulation for improved p-chloronitrobenzene (p-CNB) removal in high-salinity wastewater.
机构信息
School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, Zhejiang, China; International Science and Technology Cooperation Platform for Low-Carbon Recycling of Waste and Green Development, Zhejiang Gongshang University, Hangzhou 310018, Zhejiang, China.
School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, Zhejiang, China; International Science and Technology Cooperation Platform for Low-Carbon Recycling of Waste and Green Development, Zhejiang Gongshang University, Hangzhou 310018, Zhejiang, China; College of Environment and Resources, Zhejiang A&F University, Hangzhou 310018, Zhejiang, China.
出版信息
Sci Total Environ. 2023 Dec 20;905:167164. doi: 10.1016/j.scitotenv.2023.167164. Epub 2023 Sep 18.
As an important raw material for the synthesis of chemical and pharmaceutical, hazardous carcinogen p-chloronitrobenzene (p-CNB) has been widely found in high-salinity wastewater which need to be treated carefully. Due to the high-salinity shock on microorganisms, conventional microbial treatment technologies usually show poor effluent quality. This study initially investigated the p-CNB removal performance of microorganisms stimulated by 1.2 V low-voltage in high-salinity wastewater under facultative anaerobic conditions and further revealed the enhanced mechanisms. The results showed that the p-CNB removal kinetic parameter k in the electrostimulating microorganism reactor (EMR) increased by 104.37 % to 155.30 % compared to the microorganism reactor (MR) as the control group under the varying salinities (0-45 g/L NaCl). The secretion of extracellular polymeric substances (EPS) in halotolerant microorganisms mainly enhanced by 1.2 V voltage stimulation ranging from 0 g/L NaCl to 30 g/L NaCl. Protein concentration ratio of EMR to MR in loosely bound EPS achieved maximum value of 1.77 at the salinity of 15 g/L NaCl, and the same ratio in tightly bound EPS also peaked at 1.39 under the salinity of 30 g/L NaCl. At the salinity of 45 g/L NaCl, 1.2 V voltage stimulation mainly enhanced salt-in strategy of halotolerant microorganisms, and the intracellular Na and K concentration ratio of EMR to MR reached maximum and minimum values of 0.65 and 1.92, respectively. Furthermore, the results of microbial metagenomic and metatranscriptomic analysis showed the halotolerant microorganisms Pseudomonas_A and Nitratireductor with p-CNB removal ability were enriched significantly under 1.2 V voltage stimulation. And the gene expression of p-CNB removal, salt-in strategy and betaine transporter were enhanced under voltage stimulation at varying salinities. Our investigation provided a new solution which combined with 1.2 V voltage stimulation and halotolerant microorganisms for the treatment of high-salinity wastewater.
作为化学和制药合成的重要原料，高盐废水中广泛存在着危险致癌物质对氯硝基苯（p-CNB），需要谨慎处理。由于高盐度对微生物的冲击，传统的微生物处理技术通常表现出较差的出水质量。本研究最初在兼性厌氧条件下，研究了 1.2 V 低压刺激下微生物对高盐废水中 p-CNB 的去除性能，并进一步揭示了增强机制。结果表明，与对照组微生物反应器（MR）相比，在不同盐度（0-45 g/L NaCl）下，电刺激微生物反应器（EMR）中的 p-CNB 去除动力学参数 k 增加了 104.37%-155.30%。1.2 V 电压刺激主要增强了耐盐微生物胞外聚合物（EPS）的分泌，盐度范围从 0 g/L NaCl 到 30 g/L NaCl。EMR 与 MR 之间松散结合 EPS 中的蛋白质浓度比在 15 g/L NaCl 盐度下达到最大值 1.77，而紧密结合 EPS 中的相同比例在 30 g/L NaCl 盐度下也达到最大值 1.39。在 45 g/L NaCl 盐度下，1.2 V 电压刺激主要增强了耐盐微生物的盐进策略，EMR 与 MR 之间的细胞内 Na 和 K 浓度比达到最大值和最小值 0.65 和 1.92。此外，微生物宏基因组和宏转录组分析的结果表明，具有 p-CNB 去除能力的耐盐微生物 Pseudomonas_A 和 Nitratireductor 在 1.2 V 电压刺激下得到了显著富集。并且在不同盐度下，电压刺激增强了 p-CNB 去除、盐进策略和甜菜碱转运体的基因表达。我们的研究为处理高盐废水提供了一种新的解决方案，即将 1.2 V 电压刺激与耐盐微生物相结合。
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