Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina (UFSC) , Florianópolis, Brazil.
The Pirbright Institute , Woking, United Kingdom.
mBio. 2023 Oct 31;14(5):e0093423. doi: 10.1128/mbio.00934-23. Epub 2023 Sep 21.
One of the fundamental features that make viruses intracellular parasites is the necessity to use cellular translational machinery. Hence, this is a crucial checkpoint for controlling infections. Here, we show that dengue and Zika viruses, responsible for nearly 400 million infections every year worldwide, explore such control for optimal replication. Using immunocompetent cells, we demonstrate that arrest of protein translations happens after sensing of dsRNA and that the information required to avoid this blocking is contained in viral 5'-UTR. Our work, therefore, suggests that the non-canonical translation described for these viruses is engaged when the intracellular stress response is activated.
使病毒成为细胞内寄生虫的基本特征之一是必须使用细胞翻译机制。因此，这是控制感染的关键检查点。在这里，我们表明，每年在全球导致近 4 亿例感染的登革热和寨卡病毒会探索这种控制以实现最佳复制。使用免疫活性细胞，我们证明在检测到 dsRNA 后会发生蛋白质翻译的停滞，并且避免这种阻断所需的信息包含在病毒 5'-UTR 中。因此，我们的工作表明，当细胞内应激反应被激活时，会启动这些病毒的非典型翻译。
