Unidad de Investigaciones en Biomedicina, Zurita & Zurita Laboratorios, Quito, Ecuador.
Carrera de Ingeniería en Biotecnología, Departamento de Ciencias de la Vida, Universidad de las Fuerzas Armadas-ESPE, Sangolquí, Ecuador.
Microb Drug Resist. 2023 Nov;29(11):533-539. doi: 10.1089/mdr.2023.0125. Epub 2023 Sep 21.
In recent years, increasing resistance of to several antibiotics has been reported in different countries. The aim of this study was to evaluate the antibiotic resistance profiles of spp. isolated from clinical samples by phenotypic and molecular methods. A total of 40 nonrepetitive isolates of the group were studied from 2018 to 2019. The species was identified by API 20A system. The minimum inhibitory concentrations (MICs) were determined by Sensititre anaerobe MIC plate. The presence of the and genes was checked by conventional PCR. The association between genes and insertion sequence (IS) was performed by whole genome sequencing. Eleven isolates were categorized as metronidazole-resistant and only 2 isolates harbored the gene. Five isolates were imipenem-resistant, but gene was detected in two isolates. A gene was closely related to the A-4 allele and associated with IS614B. The gene was not related to any gene type and was considered a new variant named L. IS612 was found upstream of L gene. In view of the scarcity of data on , there is a need to surveil antibiotic resistance levels and molecular mechanisms to implement better antimicrobial therapies against this important group of bacteria.
近年来，不同国家报道了越来越多的 对几种抗生素的耐药性。本研究旨在通过表型和分子方法评估从临床样本中分离的 spp. 的抗生素耐药谱。2018 年至 2019 年，共研究了来自 2018 年至 2019 年的 40 个非重复 组分离株。通过 API 20A 系统鉴定物种。通过 Sensititre 厌氧菌 MIC 板测定最小抑菌浓度 (MIC)。通过常规 PCR 检查 和 基因的存在。通过全基因组测序检查基因和插入序列 (IS) 的关联。11 个分离株被归类为甲硝唑耐药，只有 2 个分离株携带 基因。5 个分离株对亚胺培南耐药，但在 2 个分离株中检测到 基因。A 基因与 A-4 等位基因密切相关，并与 IS614B 相关。 基因与任何 基因类型都没有关系，被认为是一种新的变体，命名为 L. 在 L 基因上游发现了 IS612。鉴于 数据稀缺，有必要监测抗生素耐药水平和分子机制，以实施针对这一重要细菌群的更好的抗菌治疗。
