Shih Y L
Jpn J Physiol. 1986;36(3):601-5. doi: 10.2170/jjphysiol.36.601.
The hyperpolarizing effect (H-effect) of d-tubocurarine on the end-plate of the isolated diaphragm pretreated with an anticholinesterase was irreversibly abolished by toosendanin (1 X 10(-5) g/ml), indicating the blockade of spontaneous non-quantal release of acetylcholine (ACh). The H-effect was also inhibited, but temporarily, when toosendanin (a dose of 0.6 LD50) was subcutaneously injected into the mouse and the diaphragm was isolated 40-120 min after injection. During such an inhibitory period, however, spontaneous release of ACh remained facilitated. It is concluded that the effect of toosendanin on non-quantal release of ACh was different from its effect on quantal release not only at the direction but also at the time course.
川楝素（1×10⁻⁵g/ml）可不可逆地消除d -筒箭毒碱对用抗胆碱酯酶预处理的离体膈肌终板的超极化作用（H效应），这表明川楝素阻断了乙酰胆碱（ACh）的自发非量子释放。当给小鼠皮下注射川楝素（剂量为0.6 LD50）并在注射后40 - 120分钟分离膈肌时，H效应也受到抑制，但只是暂时的。然而，在这种抑制期内，ACh的自发释放仍然增强。结论是，川楝素对ACh非量子释放的作用不仅在作用方向上，而且在时间进程上都不同于其对量子释放的作用。
