Guan Jing, Yang Yahan, Shan Qinghua, Zhang Haizhen, Zhou Aimin, Gong Shufang, Chai Tuanyao, Qiao Kun
College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, 150030, PR China.
College of Life Science, University of the Chinese Academy of Sciences, Beijing, 100049, PR China.
Plant Physiol Biochem. 2023 Oct;203:108043. doi: 10.1016/j.plaphy.2023.108043. Epub 2023 Sep 16.
Toxic heavy metals originating from human activities have caused irreversible harm to the environment. Toxic heavy metal ions absorbed by crop plants can seriously threaten human health. Therefore, decreasing heavy metal contents in crop plants is an urgent need. The plant cadmium resistance protein (PCR) is a heavy metal ion transporter. In this study, PePCR10 was cloned from Populus euphratica. Bioinformatics analyses revealed its transmembrane structure and gene sequence motifs. The transcript profile of PePCR10 was analyzed by RT-qPCR, and its transcript levels increased under toxic heavy metal (cadmium, lead, aluminum) treatments. Subcellular localization analyses in tobacco cells revealed that PePCR10 localizes at the plasma membrane. Compared with wild type (WT), PePCR10-overexpressing lines showed significantly higher values for plant height, root length, fresh weight, and dry weight under heavy metal stress. Electrolyte leakage, nitroblue tetrazolium staining, and chlorophyll fluorescence analyses indicated that Cd/Al tolerance in PePCR10-overexpressing lines was stronger than that in WT. The Cd/Al contents were lower in the PePCR10-overexpressing lines than in WT under Cd/Al stress. Our results show that PePCR10 can reduce the heavy metal content in poplar and enhance its Cd/Al tolerance. Hence, PePCR10 is a candidate genetic resource for effectively reducing heavy metal accumulation in crops.
源自人类活动的有毒重金属已对环境造成不可逆转的危害。作物吸收的有毒重金属离子会严重威胁人类健康。因此，降低作物中的重金属含量迫在眉睫。植物镉抗性蛋白（PCR）是一种重金属离子转运蛋白。在本研究中，从胡杨中克隆了PePCR10。生物信息学分析揭示了其跨膜结构和基因序列基序。通过RT-qPCR分析了PePCR10的转录谱，其转录水平在有毒重金属（镉、铅、铝）处理下升高。烟草细胞中的亚细胞定位分析表明，PePCR10定位于质膜。与野生型（WT）相比，在重金属胁迫下，过表达PePCR10的株系在株高、根长、鲜重和干重方面的值显著更高。电解质渗漏、氮蓝四唑染色和叶绿素荧光分析表明，过表达PePCR10的株系对镉/铝的耐受性强于野生型。在镉/铝胁迫下，过表达PePCR10的株系中镉/铝含量低于野生型。我们的结果表明，PePCR10可以降低杨树中的重金属含量并增强其对镉/铝的耐受性。因此，PePCR10是有效降低作物中重金属积累的候选遗传资源。
