CALB1：鸡回肠黏液中的一种新型抗病毒因子。

CALB1: Anovel antiviral factor in chicken ileal mucus.

机构信息

College of Life Sciences, SanYa Institute of Nanjing Agricultural University, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu 210095, PR China.

College of Veterinary medicine, Nanjing Agricultural University, Wei gang 1, Nanjing, Jiangsu 210095, PR China.

出版信息

Int J Biol Macromol. 2023 Dec 31;253(Pt 3):127007. doi: 10.1016/j.ijbiomac.2023.127007. Epub 2023 Sep 19.

DOI:10.1016/j.ijbiomac.2023.127007

PMID:37734520

Abstract

Intestinal mucus is the first line of defense against pathogens and has several active components. Poultry have a short intestine, the mucus of which may contain antiviral components. We hence investigated the antiviral components of mucus and explored their mechanisms of action. Initially, we isolated chicken intestinal mucus proteins that significantly inhibited the replication of avian viruses. The ileum 10-30 kDa protein fraction showed the greatest inhibition of viral replication. Moreover, liquid chromatography-mass spectrometry revealed 12 high-abundance proteins in the ileum 10-30 kDa protein fraction. Among them, we investigated the antiviral activity of calcium binding protein 1 (CALB1). Furthermore, eukaryotically and prokaryotically expressed CALB1 significantly suppressed the replication of avian viruses, possibly by binding calcium ions and/or inducing autophagy. In conclusion, we isolated and identified CALB1 from chicken intestinal mucus, which suppressed replication of avian viruses by regulating cellular calcium-ion homeostasis and autophagy.

摘要

肠黏液是抵御病原体的第一道防线，具有多种活性成分。家禽的肠道较短，其黏液可能含有抗病毒成分。因此，我们研究了黏液中的抗病毒成分，并探讨了其作用机制。首先，我们分离出了能显著抑制禽病毒复制的鸡肠黏液蛋白。回肠 10-30 kDa 蛋白部分对病毒复制的抑制作用最大。此外，液相色谱-质谱分析显示回肠 10-30 kDa 蛋白部分含有 12 种高丰度蛋白。其中，我们研究了钙结合蛋白 1 (CALB1) 的抗病毒活性。此外，真核和原核表达的 CALB1 显著抑制了禽病毒的复制，可能是通过结合钙离子和/或诱导自噬。总之，我们从鸡肠黏液中分离并鉴定了 CALB1，它通过调节细胞内钙离子稳态和自噬来抑制禽病毒的复制。