National Key Laboratory of Green Pesticide, South China Agricultural University, Guangzhou 510642, China.
College of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 4):126947. doi: 10.1016/j.ijbiomac.2023.126947. Epub 2023 Sep 19.
A chitosan-based nanoparticle was prepared using chitosan (CS) and O-carboxymethyl chitosan (O-CMCS). Our study revealed that chitosan/O-carboxymethyl chitosan/tebuconazole nanoparticles (CS/O-CMCS/TBA NPs) exhibited superior antifungal activity, foliar adhesion, and microbial target adhesion performance compared to commercial suspension concentrate (SC). The antifungal activity of CS/O-CMCS/TBA NPs against C. gloeosporioides, with a 3.13-fold increase in efficacy over TBA (SC). We also found that low concentrations of CS/O-CMCS NPs promoted the growth of C. gloeosporioides and enhanced the fungal catabolism of chitosan. Overall, the CS/O-CMCS/TBA NPs were found to possess the remarkable capability to selectively aggregate around pathogenic microorganisms and CS/O-CMCS NPs can enhance the fungal catabolism of chitosan. CS/O-CMCS/TBA NPs, as a "sugar-coated bomb", was a promising asset for effective plant disease management and pesticide utilization through the affinity of chitosan-based nanoparticles and C. gloeosporioides, enabling targeted delivery and targeted release of their encapsulated active ingredient, which was important for the development and application of biocompatible chitosan-based nanopesticides.
采用壳聚糖（CS）和 O-羧甲基壳聚糖（O-CMCS）制备了一种基于壳聚糖的纳米粒子。我们的研究表明，壳聚糖/O-羧甲基壳聚糖/戊唑醇纳米粒子（CS/O-CMCS/TBA NPs）与商业悬浮剂（SC）相比，表现出优异的抗真菌活性、叶面附着力和微生物靶标附着力。CS/O-CMCS/TBA NPs 对胶孢炭疽菌的抗真菌活性比 TBA（SC）提高了 3.13 倍。我们还发现低浓度的 CS/O-CMCS NPs 促进了胶孢炭疽菌的生长，并增强了壳聚糖的真菌分解代谢。总的来说，CS/O-CMCS/TBA NPs 具有选择性聚集在致病微生物周围的显著能力，CS/O-CMCS NPs 可以增强壳聚糖的真菌分解代谢。CS/O-CMCS/TBA NPs 作为一种“糖衣炸弹”，通过壳聚糖纳米粒子与胶孢炭疽菌的亲和力，为有效进行植物病害管理和农药利用提供了一种很有前途的方法，能够实现其封装活性成分的靶向输送和靶向释放，这对于生物相容的壳聚糖基纳米农药的开发和应用很重要。
