Department of Clinical Pharmacology, Xiangya Hospital, Central South University, China; Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, China; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, China; National Clinical Research Center for Geriatric Disorders, China.
Department of Clinical Pharmacology, Xiangya Hospital, Central South University, China; Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, China; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, China; National Clinical Research Center for Geriatric Disorders, China; Department of Pharmacy, The Central Hospital of Shaoyang, Shaoyang, China.
Microbes Infect. 2024 Jan-Feb;26(1-2):105228. doi: 10.1016/j.micinf.2023.105228. Epub 2023 Sep 20.
It has recently been found that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) m6A modifications can affect viral replication and function. However, no studies to date have shown a correlation between SARS-CoV-2 m6A modifications and viral pathogenicity. In this study, we analyzed m6A modification in 2,190,667 SARS-CoV-2 genomic RNAs. m6A modifications of SARS-CoV-2 from different lineages, causing mild or severe COVID-19 and showing breakthrough for different vaccines were analyzed to explore correlations with viral pathogenicity. The results suggested that the presence of more m6A modifications in the SARS-CoV-2 N region (positive strand) correlates with weaker pathogenicity. In addition, we identified three m6A modification sites correlating with weak pathogenicity (924 in ORF1ab, 15,659 in ORF1ab, 28,288 in N, 28,633 in N and 29,385 in N, 29,707 in 3'UTR) and one with strong pathogenicity (74 in 5'UTR). These results provide new information for understanding the prevalence of SARS-CoV-2 and controlling the virus.
最近发现，严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）m6A 修饰可以影响病毒复制和功能。然而，迄今为止尚无研究表明 SARS-CoV-2 m6A 修饰与病毒致病性之间存在相关性。在这项研究中，我们分析了 2,190,667 个 SARS-CoV-2 基因组 RNA 的 m6A 修饰。分析了来自不同谱系的 SARS-CoV-2 的 m6A 修饰，这些病毒引起轻度或重度 COVID-19，并对不同疫苗表现出突破性，以探索与病毒致病性的相关性。结果表明，SARS-CoV-2 N 区域（正链）中存在更多的 m6A 修饰与较弱的致病性相关。此外，我们还鉴定了三个与弱致病性相关的 m6A 修饰位点（ORF1ab 中的 924 位、ORF1ab 中的 15,659 位、N 中的 28,288 位、N 中的 28,633 位、N 中的 29,385 位和 3'UTR 中的 29,707 位）和一个与强致病性相关的 m6A 修饰位点（5'UTR 中的 74 位）。这些结果为了解 SARS-CoV-2 的流行情况和控制病毒提供了新的信息。
