Ruwoldt Jost, Dørdal Helgheim Marita, Tanase-Opedal Mihaela, Syverud Kristin
RISE PFI AS, Høgskoleringen 6B, 7491 Trondheim, Norway.
Data Brief. 2023 Sep 6;50:109549. doi: 10.1016/j.dib.2023.109549. eCollection 2023 Oct.
This article provides UV-spectrophotometry data of technical lignin samples in solutions, which were acquired after ambient aging for up to 110 days or looped measurements on fresh solutions. UV-spectrophotometry of lignin is a useful technique, as it can a) quantify the concentration and purity of lignin in a given sample, b) determine the abundance of phenolic hydroxyl groups, and c) yield qualitative information about chemical modification of the lignin macromolecule. In addition, the technique is rapid and easy to use. Still, solutions of lignin are known to be unstable; in particular at high pH or in presence of UV-light. The data in this article may hence serve as guide in the experimental conduct and design, as it shows the reproducibility of UV-spectrophotometry measurements of lignin. Stock solutions of technical lignin were made according to previously published procedure [1]. The solutions in dimethyl sulfoxide (DMSO) were aged in 100 mL volumetric flasks with glass stopper, taking periodic samples for measurements in a Shimadzu UV-1900 UV-vis spectrophotometer. The instrument recorded the spectrum from 500 to 200 nm at 1.0 nm intervals and medium speed, using quartz cuvettes with a pathlength of 1 cm. In addition, looped measurements were conducted on fresh solutions, where the instrument repeated the spectral range of 500 to 200 nm for in total sixteen times. The latter examined solutions of technical lignin in DMSO solvent as well as in 0.2 N NaOH in water.
本文提供了工业木质素样品在溶液中的紫外分光光度数据，这些数据是在环境老化长达110天之后或对新鲜溶液进行循环测量后获得的。木质素的紫外分光光度法是一项有用的技术，因为它可以：a）量化给定样品中木质素的浓度和纯度；b）确定酚羟基的丰度；c）提供有关木质素大分子化学修饰的定性信息。此外，该技术快速且易于使用。不过，已知木质素溶液不稳定，特别是在高pH值或有紫外光存在的情况下。因此，本文中的数据可作为实验操作和设计的指南，因为它展示了木质素紫外分光光度测量的可重复性。工业木质素储备溶液是按照先前发表的程序[1]制备的。二甲基亚砜（DMSO）溶液在带玻璃塞的100 mL容量瓶中老化，定期取样在岛津UV-1900紫外可见分光光度计中进行测量。该仪器使用光程为1 cm的石英比色皿，以1.0 nm的间隔和中等速度记录500至200 nm的光谱。此外，对新鲜溶液进行了循环测量，仪器在500至200 nm的光谱范围内总共重复测量了16次。后者检测了工业木质素在DMSO溶剂以及在水中0.2 N氢氧化钠中的溶液。
