抹茶提取物对 MCF-7 乳腺癌细胞活力和雌激素受体-β表达的影响。
Effects of matcha tea extract on cell viability and estrogen receptor-β expression on MCF-7 breast cancer cells.
机构信息
Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Marchioninistr. 15, 81377, Munich, Germany.
Department of Urology and Transplant Surgery, Klinikum Stuttgart, Stuttgart, Germany.
出版信息
Arch Gynecol Obstet. 2024 Apr;309(4):1509-1514. doi: 10.1007/s00404-023-07209-z. Epub 2023 Sep 22.
PURPOSE
In the following work, we investigated the effect of matcha green tea extract (MTE) on MCF-7 breast cancer cell viability and estrogen receptor-beta expression (ERβ).
METHODS
MCF-7 cells were stimulated with MTE at concentrations of 5 and 10 µg/ml. Cell viability was assessed using a water-soluble tetrazolium assay (WST-1 assay) after an incubation time of 72 h. ERβ was quantified at gene level by real-time polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level.
RESULTS
The WST-1 test showed a significant inhibition of viability in MFC-7 cells after 72 h at 10 µg/ml. The WB demonstrated a significant quantitative decrease of ERβ at protein level with MTE concentrations of 10 µg/ml. In contrast, the PCR did not result in significant downregulation of ERβ.
CONCLUSION
MTE decreases the cell viability of MCF-7 cells and furthermore leads to a decrease of ERβ at protein level.
目的
在本项工作中，我们研究了抹茶绿茶提取物（MTE）对 MCF-7 乳腺癌细胞活力和雌激素受体-β表达（ERβ）的影响。
方法
MCF-7 细胞用浓度为 5 和 10 μg/ml 的 MTE 刺激。孵育 72 h 后，通过水溶性四唑盐（WST-1 测定）评估细胞活力。实时聚合酶链反应（PCR）用于在基因水平上定量 ERβ。进行蛋白质印迹（WB）以定性评估表达行为。
结果
WST-1 试验显示，在 10 μg/ml 时，MCF-7 细胞在 72 h 后活力显著抑制。WB 显示，用 10 μg/ml 的 MTE 处理后，ERβ 的蛋白水平显著减少。相比之下，PCR 并未导致 ERβ 的显著下调。
结论
MTE 降低 MCF-7 细胞的活力，并进一步导致 ERβ 蛋白水平的降低。
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