筛选果蝇兼性孤雌生殖的方案。
Protocol for screening facultative parthenogenesis in Drosophila.
机构信息
Department of Genetics, University of Cambridge, Cambridge, CB2 3EH Cambridgeshire, UK.
Department of Genetics, University of Cambridge, Cambridge, CB2 3EH Cambridgeshire, UK; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
出版信息
STAR Protoc. 2023 Dec 15;4(4):102585. doi: 10.1016/j.xpro.2023.102585. Epub 2023 Sep 22.
Most species of sexually reproducing Drosophila are capable of some degree of facultative parthenogenesis, which involves the initiation of development in an unfertilized egg. Here, we present an optimized protocol to screen facultative parthenogenesis in Drosophila. We describe steps for the collection and maintenance of virgin flies. We then detail offspring screening for the analysis of parthenogenesis. This protocol can be applied to different Drosophila strains and can be adapted for the analysis of parthenogenesis in other animals. For complete details on the use and execution of this protocol, please refer to Sperling et al..
大多数有性生殖的果蝇物种都具有一定程度的兼性孤雌生殖能力，这种生殖方式涉及到未受精卵的发育启动。在这里，我们提供了一个优化的方案来筛选果蝇的兼性孤雌生殖。我们描述了收集和维持处女蝇的步骤。然后，我们详细介绍了后代筛选，以分析孤雌生殖。本方案可应用于不同的果蝇品系，并可适用于其他动物孤雌生殖的分析。如需详细了解本方案的使用和执行，请参考 Sperling 等人的研究。
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