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研究寄生蜂对果蝇长期影响的实验方案。

Protocol for studying parasitoid-induced long-term effects in Drosophila.

作者信息

Sadanandappa Madhumala K, Bosco Giovanni

机构信息

Department of Molecular and Systems Biology, Geisel School of Medicine at Dartmouth, Hanover, NH 03755, USA.

出版信息

STAR Protoc. 2024 Dec 20;5(4):103438. doi: 10.1016/j.xpro.2024.103438. Epub 2024 Nov 4.

DOI:10.1016/j.xpro.2024.103438
PMID:39499614
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11568782/
Abstract

Drosophila and its parasitoids provide an ecologically relevant model for studying host-parasitoid biology, focusing on the behavioral and physiological responses involved in host defensive strategies and parasitoid countermeasures. Here, we outline a protocol for rearing Pachycrepoideus, a pupal parasitoid wasp, and a behavioral assay to assess the long-term impact of parasitoid exposure on adult Drosophila. We detail the steps for preparing and cohabiting Drosophila with the wasps, documenting egg-laying, and analyzing reproductive responses and eclosion in fruit flies. For complete details on the use and execution of this protocol, please refer to Sadanandappa et al..

摘要

果蝇及其寄生蜂为研究宿主 - 寄生蜂生物学提供了一个具有生态相关性的模型，重点关注宿主防御策略和寄生蜂对策中涉及的行为和生理反应。在此，我们概述了一种饲养蛹寄生蜂厚结猛蚁茧蜂的方法以及一种行为测定法，以评估寄生蜂暴露对成年果蝇的长期影响。我们详细介绍了准备果蝇并使其与黄蜂同居、记录产卵情况以及分析果蝇繁殖反应和羽化情况的步骤。有关此方法的使用和执行的完整详细信息，请参考萨达南达帕等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/147d/11568782/b3afd3f280f2/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/147d/11568782/baf0a583411b/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/147d/11568782/0f5ecbe7124e/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/147d/11568782/a5e2a45f9833/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/147d/11568782/49e83fc181a9/sc1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/147d/11568782/49f734810456/sc2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/147d/11568782/60eb39afbcd7/sc3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/147d/11568782/59614e142bc9/sc4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/147d/11568782/b3afd3f280f2/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/147d/11568782/baf0a583411b/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/147d/11568782/0f5ecbe7124e/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/147d/11568782/a5e2a45f9833/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/147d/11568782/49e83fc181a9/sc1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/147d/11568782/49f734810456/sc2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/147d/11568782/60eb39afbcd7/sc3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/147d/11568782/59614e142bc9/sc4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/147d/11568782/b3afd3f280f2/gr3.jpg

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Cell Rep. 2024 Jan 23;43(1):113657. doi: 10.1016/j.celrep.2023.113657. Epub 2024 Jan 3.
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Parasitoid Wasp Culturing and Assay to Study Parasitoid-induced Reproductive Modifications in .用于研究寄生蜂诱导的[具体对象]生殖变化的寄生蜂培养与测定
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Search performance and octopamine neuronal signaling mediate parasitoid induced changes in Drosophila oviposition behavior.搜索性能和章鱼胺神经元信号介导寄生蜂诱导的果蝇产卵行为变化。
Nat Commun. 2022 Aug 2;13(1):4476. doi: 10.1038/s41467-022-32203-5.
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DROP: Molecular voucher database for identification of Drosophila parasitoids.虫寄生蜂分子凭证数据库
Mol Ecol Resour. 2021 Oct;21(7):2437-2454. doi: 10.1111/1755-0998.13435. Epub 2021 Jun 10.
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