Cheng Siren, Liu Haidong, Li Keqing, Zheng Likai, Su Meilin, Lin Xueer, Huang Guobao, Ren Yong
Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology, Yulin Normal University, Yulin, China.
College of Biology and Pharmacy, Yulin Normal University, Yulin, China.
J Sci Food Agric. 2024 Jan 30;104(2):1178-1189. doi: 10.1002/jsfa.13004. Epub 2023 Oct 31.
Riboflavin, a vital water-soluble vitamin with antioxidative activity, plays a critical role in maintaining overall bodily health and defense responses. However, its impact on fragrant rice yield and aroma remains unexplored.
In a 2022 pot experiment with Meixiangzhan and Yuxiangyouzhan fragrant rice cultivars, we applied riboflavin foliar treatments at concentrations of 0 (CK), 10 (R10), 20 (R20), and 40 (R40) mg L during the initial heading stage. Riboflavin increased rice yield, 2-acetyl-1-pyrroline (2-AP) content, and antioxidative properties. It boosted 2-AP level by 13.1-50.1% for Meixiangzhan and 22.3-35.3% for Yuxiangyouzhan, with the highest levels in R20 and R10 treatments. This increase is significantly correlated with elevated levels of proline, pyrroline-5-carboxylic acid, pyrroline, and methylglyoxal, as well as heightened enzyme activities, including those of proline dehydrogenase, ornithine aminotransferase, and pyrroline-5-carboxylic acid synthetase (P5CS). The R20 treatment resulted in the highest yield due to an improved seed-setting rate. Importantly, a positive correlation emerged between 2-AP content and yield, both significantly linked to superoxide dismutase, proline, hydrogen peroxide, P5CS, catalase, and pyrroline.
Riboflavin maintained enzyme activities, regulated substance synthesis pathways, and increased 2-AP and yield, especially in the R20 treatment. These insights advance fragrant rice production theory by uncovering riboflavin's role in the development of fragrant rice. © 2023 Society of Chemical Industry.
核黄素是一种具有抗氧化活性的重要水溶性维生素，在维持整体身体健康和防御反应中起着关键作用。然而，其对香稻产量和香气的影响尚未得到探索。
在2022年对美香占和玉香油占香稻品种进行的盆栽试验中，我们在始穗期分别以0（CK）、10（R10）、20（R20）和40（R40）mg·L的浓度进行核黄素叶面处理。核黄素提高了水稻产量、2-乙酰-1-吡咯啉（2-AP）含量和抗氧化性能。美香占的2-AP水平提高了13.1% - 50.1%，玉香油占提高了22.3% - 35.3%，在R20和R10处理中达到最高水平。这种增加与脯氨酸、吡咯啉-5-羧酸、吡咯啉和甲基乙二醛水平的升高以及包括脯氨酸脱氢酶、鸟氨酸转氨酶和吡咯啉-5-羧酸合成酶（P5CS）在内的酶活性增强显著相关。由于结实率提高，R20处理的产量最高。重要的是，2-AP含量与产量之间呈现正相关，两者均与超氧化物歧化酶、脯氨酸、过氧化氢、P5CS、过氧化氢酶和吡咯啉显著相关。
核黄素维持酶活性，调节物质合成途径，并提高2-AP含量和产量，尤其是在R20处理中。这些见解通过揭示核黄素在香稻发育中的作用，推进了香稻生产理论。© 2023化学工业协会。
