Kölle Martina, Crivelente Horta Maria Augusta, Benz J Philipp, Pilgård Annica
Chair of Wood Science, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Munich, Germany.
Professorship of Fungal Biotechnology in Wood Science, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Munich, Germany.
Front Fungal Biol. 2021 Sep 6;2:701579. doi: 10.3389/ffunb.2021.701579. eCollection 2021.
Brown rot fungi degrade wood in a two-step process in which enzymatic hydrolysis is preceded by an oxidative degradation phase. While a detailed understanding of the molecular processes during brown rot decay is mandatory for being able to better protect wooden products from this type of degradation, the underlying mechanisms are still not fully understood. This is particularly true for wood that has been treated to increase its resistance against rot. In the present study, the two degradation phases were separated to study the impact of wood acetylation on the behavior of three brown rot fungi commonly used in wood durability testing. Transcriptomic data from two strains of (FPRL280 and MAD-698) and were recorded to elucidate differences between the respective decay strategies. Clear differences were found between the two decay stages in all fungi. Moreover, strategies varied not only between species but also between the two strains of the same species. The responses to wood acetylation showed that decay is generally delayed and that parts of the process are attenuated. By hierarchical clustering, we could localize several transcription factors within gene clusters that were heavily affected by acetylation, especially in . The results suggest that regulatory circuits evolve rapidly and are probably the major cause behind the different decay strategies as observed even between the two strains of . Identifying key genes in these processes can help in decay detection and identification of the fungi by biomarker selection, and also be informative for other fields, such as fiber modification by biocatalysts and the generation of biochemical platform chemicals for biorefinery applications.
褐腐真菌通过两步过程降解木材，其中在酶促水解之前有一个氧化降解阶段。虽然要更好地保护木制品免受此类降解，必须详细了解褐腐腐朽过程中的分子过程，但潜在机制仍未完全明了。对于经过处理以提高其抗腐性的木材来说尤其如此。在本研究中，将两个降解阶段分开，以研究木材乙酰化对木材耐久性测试中常用的三种褐腐真菌行为的影响。记录了来自两种菌株（FPRL280和MAD - 698）和的转录组数据，以阐明各自腐朽策略之间的差异。在所有真菌的两个腐朽阶段之间发现了明显差异。此外，策略不仅在物种之间不同，而且在同一物种的两种菌株之间也不同。对木材乙酰化的反应表明，腐朽通常会延迟，并且该过程的部分环节会减弱。通过层次聚类，我们可以在受乙酰化严重影响的基因簇中定位几个转录因子，特别是在中。结果表明，调控回路进化迅速，可能是观察到的即使在两种菌株之间也存在不同腐朽策略的主要原因背后的因素。识别这些过程中的关键基因有助于通过生物标志物选择进行腐朽检测和真菌鉴定，并且对其他领域也有参考价值，例如生物催化剂对纤维的改性以及用于生物炼制应用的生化平台化学品的生成。
