Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, Japan.
Biosci Biotechnol Biochem. 2021 Jun 24;85(7):1782-1788. doi: 10.1093/bbb/zbab080.
Brown rot fungi show a two-step wood degradation mechanism comprising oxidative radical-based and enzymatic saccharification systems. Recent studies have demonstrated that the brown rot fungus Rhodonia placenta expresses oxidoreductase genes ahead of glycoside hydrolase genes and spatially protects the saccharification enzymes from oxidative damage of the oxidoreductase reactions. This study aimed to assess the generality of the spatial gene regulation of these genes in other brown rot fungi and examine the effects of carbon source on the gene regulation. Gene expression analysis was performed on 14 oxidoreductase and glycoside hydrolase genes in the brown rot fungus Gloeophyllum trabeum, directionally grown on wood, sawdust-agar, and glucose-agar wafers. In G. trabeum, both oxidoreductase and glycoside hydrolase genes were expressed at higher levels in sections behind the wafers. The upregulation of glycoside hydrolase genes was significantly higher in woody substrates than in glucose, whereas the oxidoreductase gene expression was not affected by substrates.
褐腐真菌表现出两步式木材降解机制，包括氧化自由基和酶糖化系统。最近的研究表明，褐腐菌 Rhodonia placenta 在糖苷水解酶基因之前表达氧化还原酶基因，并在空间上保护糖化酶免受氧化还原酶反应的氧化损伤。本研究旨在评估这些基因在其他褐腐真菌中的空间基因调控的普遍性，并研究碳源对基因调控的影响。在定向生长在木块、木屑-琼脂和葡萄糖-琼脂片上的褐腐菌 Gloeophyllum trabeum 中，对 14 个氧化还原酶和糖苷水解酶基因进行了基因表达分析。在 G. trabeum 中，氧化还原酶和糖苷水解酶基因在片材后面的切片中表达水平更高。糖苷水解酶基因在木质基质中的上调表达显著高于葡萄糖，而氧化还原酶基因的表达不受基质的影响。
