Qingdao University, Qingdao, China.
Guangzhou Kingmed Diagnostics Group, Guangzhou, China.
PeerJ. 2023 Sep 19;11:e16057. doi: 10.7717/peerj.16057. eCollection 2023.
Our study aims to investigate the long non-coding RNA plasmacytoma variant translocation 1 (lncRNA PVT1) in lower extremity arteriosclerosis obliterans (LEASO) patient serum and its clinical significance in LEASO.
From July 2021 to April 2022, 133 LEASO patients diagnosed at the Qingdao Municipal Hospital were included. Among them, 44 complicated with coronary artery disease (CAD) were classified as the LEASO with CAD group. The remaining 89 were marked as the LEASO group, which was classified into single ( = 48) and double ( = 41) lower limb groups, with the former being subclassified into the left ( = 28) and right ( = 20) lower limb groups based on the affected sites. Fifty healthy individuals who came to our hospital for physical examination during the same period were randomly included and defined as the Healthy Control group. PVT1 expression was detected in serum samples from each group using a quantitative reverse transcriptase-polymerase chain reaction , and differences in expression levels were calculated. The ankle-brachial index (ABI) of patients in the LEASO group was measured using a sphygmomanometer, and its correlation with PVT1 was analyzed. Clinical data and laboratory test results (including blood routine, liver and renal function, and blood lipids) were collected for all patients upon admission. Logistic regression analyses were performed to determine the influence of PVT1 and laboratory test results on LEASO. The diagnosis and prediction of LEASO were obtained by combing PVT1 with laboratory test indicators.
It was found that lncRNA PVT1 expression was the highest in the serum of the LEASO with CAD group, followed by the LEASO and control groups ( < 0.05). Within the LEASO group, no significant difference in PVT1 expression was seen between the left and right limbs ( > 0.05), nor between the single and double lower limb groups. Furthermore, the PVT1 expression increased with the Rutherford grades, indicating a negative correlation between PVT1 and ABI. Logistic regression analysis revealed that triglycerides (OR = 2.972, 95% CI [1.159-7.618]), cholesterol (OR = 6.655, 95% CI [1.490-29.723]), C-reactive protein (OR = 1.686, 95% CI [1.218-2.335]), and PVT1 (OR = 2.885, 95% CI [1.350-6.167]) were independent risk factors for LEASO. Finally, strong sensitivity was observed in the receiver operating characteristic curve when combining PVT1 with meaningful laboratory indicators to diagnose and predict LEASO.
lncRNA PVT1 promotes LEASO occurrence and progression and is related to atherosclerosis severity. The expression of PVT1 was negatively correlated with ABI. Logistic regression analysis suggested that blood lipid levels and inflammatory reactions might be related to LEASO occurrence. PVT1 was incorporated into laboratory indicators to predict LEASO. The subject's working curve area was large, and the prediction results were highly sensitive.
本研究旨在探讨浆细胞瘤变异易位 1（lncRNA PVT1）在下肢动脉硬化闭塞症（LEASO）患者血清中的表达及其在 LEASO 中的临床意义。
纳入 2021 年 7 月至 2022 年 4 月在青岛市立医院确诊的 133 例 LEASO 患者，其中 44 例合并冠状动脉疾病（CAD）患者分为 LEASO 合并 CAD 组。其余 89 例为 LEASO 组，分为单（=48）和双（=41）下肢组，根据受累部位将前者分为左（=28）和右（=20）下肢组。同期随机纳入 50 名在我院体检的健康个体作为健康对照组。采用实时荧光定量逆转录聚合酶链反应检测各组血清中 PVT1 的表达，并计算其表达水平的差异。使用血压计测量 LEASO 组患者的踝肱指数（ABI），并分析其与 PVT1 的相关性。入院时收集所有患者的临床资料和实验室检查结果（包括血常规、肝肾功能和血脂）。采用 Logistic 回归分析确定 PVT1 和实验室检查结果对 LEASO 的影响。通过结合 PVT1 和实验室检查指标，获得 LEASO 的诊断和预测结果。
结果发现，LEASO 合并 CAD 组患者血清中 lncRNA PVT1 的表达最高，其次是 LEASO 组和对照组（<0.05）。在 LEASO 组中，PVT1 表达在左、右下肢之间（>0.05）或单、双下肢组之间无显著差异。此外，PVT1 表达随着 Rutherford 分级的增加而增加，提示 PVT1 与 ABI 呈负相关。Logistic 回归分析显示，甘油三酯（OR=2.972，95%CI[1.159-7.618]）、胆固醇（OR=6.655，95%CI[1.490-29.723]）、C 反应蛋白（OR=1.686，95%CI[1.218-2.335]）和 PVT1（OR=2.885，95%CI[1.350-6.167]）是 LEASO 的独立危险因素。最后，当结合 PVT1 和有意义的实验室指标来诊断和预测 LEASO 时，受试者工作特征曲线显示出较强的敏感性。
lncRNA PVT1 促进 LEASO 的发生和发展，与动脉粥样硬化的严重程度有关。PVT1 的表达与 ABI 呈负相关。Logistic 回归分析表明，血脂水平和炎症反应可能与 LEASO 的发生有关。将 PVT1 纳入实验室指标可预测 LEASO。受试者工作曲线的面积较大，预测结果具有较高的敏感性。
