Romanowski Eric G, Brothers Kimberly M, Calvario Rachel C, Stella Nicholas A, Kim Tami, Elsayed Mennat, Kadouri Daniel E, Shanks Robert M Q
Charles T. Campbell Laboratory of Ophthalmic Microbiology, Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA.
Department of Oral Biology, Rutgers School of Dental Medicine, Newark, NJ.
bioRxiv. 2023 Sep 19:2023.09.17.558130. doi: 10.1101/2023.09.17.558130.
Endogenous endophthalmitis caused by Gram-negative bacteria is an intra-ocular infection that can rapidly progress to irreversible loss of vision. While most endophthalmitis isolates are susceptible to antibiotic therapy, the emergence of resistant bacteria necessitates alternative approaches to combat intraocular bacterial proliferation. In this study the ability of predatory bacteria to limit intraocular growth of , , and was evaluated in a New Zealand White rabbit endophthalmitis prevention model. Predatory bacteria and were able to reduce proliferation of keratitis isolates of and . However, it was not able to significantly reduce which is not a productive prey for these predatory bacteria, suggesting that the inhibitory effect on requires active predation rather than an antimicrobial immune response. Similarly, UV-inactivated were unable to prevent proliferation of . Together, these data suggest predation of Gram-negative bacteria within the intra-ocular environment.
革兰氏阴性菌引起的内源性眼内炎是一种可迅速发展为不可逆视力丧失的眼内感染。虽然大多数眼内炎分离株对抗生素治疗敏感，但耐药菌的出现需要采用替代方法来对抗眼内细菌增殖。在本研究中，在新西兰白兔眼内炎预防模型中评估了捕食性细菌限制、和眼内生长的能力。捕食性细菌和能够减少角膜炎分离株和的增殖。然而，它不能显著减少，因为对于这些捕食性细菌来说它不是有效的猎物，这表明对的抑制作用需要主动捕食而不是抗菌免疫反应。同样，紫外线灭活的也无法阻止的增殖。这些数据共同表明眼内环境中革兰氏阴性菌存在捕食现象。
