Karl Landsteiner University of Health Sciences; Department of Neurosurgery, University Hospital St. Pölten; Cerebrovascular Research Group, Department of Neurosurgery, University Hospital St. Pölten.
Division of Biomedical Research, Medical University of Vienna.
J Vis Exp. 2023 Sep 8(199). doi: 10.3791/63738.
Giant aneurysms are dangerous lesions requiring endovascular treatment, with high rates of aneurysm recanalization and re-rupture. Reliable in vivo models are rare but are required for testing new endovascular devices. We demonstrate the technical aspects of the creation of giant bifurcation aneurysms in New Zealand white rabbits (2.5-5.5 kg). A 25-30 mm long venous pouch is taken from the external jugular vein, and a bifurcation between both carotid arteries is created microsurgically. The pouch is sutured in the bifurcation to mimic a giant aneurysm. This protocol summarizes our previously published standard technique for venous pouch true arterial bifurcation aneurysms and highlights its essential modification steps for giant aneurysms. Using this modified technique, we were able to create an animal model for giant aneurysms with high comparability to humans regarding the hemodynamics and coagulation systems. Furthermore, low morbidity and high aneurysm patency rates were achieved. The proposed giant aneurysm model offers an excellent possibility for testing new endovascular devices.
巨大动脉瘤是一种危险的病变，需要进行血管内治疗，其动脉瘤再通和再破裂的发生率很高。可靠的体内模型很少见，但对于测试新的血管内设备是必需的。我们展示了在新西兰白兔（2.5-5.5 公斤）中创建巨大分叉动脉瘤的技术方面。从颈外静脉取出 25-30 毫米长的静脉袋，并通过显微手术在颈总动脉之间创建分叉。将静脉袋缝合在分叉处以模拟巨大动脉瘤。该方案总结了我们之前发表的静脉袋真动脉分叉动脉瘤的标准技术，并强调了其用于巨大动脉瘤的基本修改步骤。使用这种改良技术，我们能够创建一个与人类在血液动力学和凝血系统方面具有高度可比性的巨大动脉瘤动物模型。此外，还实现了低发病率和高动脉瘤通畅率。所提出的巨大动脉瘤模型为测试新的血管内设备提供了极好的可能性。
