Key Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Key Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Food Chem. 2024 Feb 15;434:137559. doi: 10.1016/j.foodchem.2023.137559. Epub 2023 Sep 21.
High moisture content of fresh bee pollen makes it difficult to preserve and thus makes drying a necessary process during the bee pollen production. Drying treatment will affect its quality and the effects of sun drying, hot-air drying and freeze drying on the proteome of rape (Brassica napus) bee pollen have been evaluated using label-free quantitative proteomics by liquid chromatography-tandem mass spectrometer (LC-MS/MS). A total of 8377 proteins are identified, among which the most abundant differential proteins were found in freeze drying-treated samples. Also freeze-drying treatment maximizes the content of antioxidant, antibacterial and anemic bioactive pollen protein. Besides, rape bee pollen is found to adjust its metabolism to protect itself during the drying process. These results can be favorable to evaluate the effects of drying treatment on the nutrition and function of processed rape bee pollen and insight into how rape bee pollen proteins respond to dehydration.
新鲜蜂花粉水分含量高，难以保存，因此在蜂花粉生产过程中干燥是必要的。干燥处理会影响其质量，本研究采用无标记定量蛋白质组学方法，通过液相色谱-串联质谱（LC-MS/MS）技术评估了自然晾晒、热风干燥和冷冻干燥对油菜（Brassica napus）蜂花粉蛋白质组的影响。共鉴定到 8377 种蛋白质，其中冷冻干燥处理样品中差异表达蛋白最丰富。此外，冷冻干燥处理最大限度地提高了抗氧化、抗菌和贫血生物活性花粉蛋白的含量。此外，油菜蜂花粉在干燥过程中会调整其代谢以保护自身。这些结果有利于评估干燥处理对加工油菜蜂花粉的营养和功能的影响，并深入了解油菜蜂花粉蛋白如何应对脱水。
