National Key Laboratory of Green Pesticide, South China Agricultural University, Guangzhou, China; Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, China; Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture, South China Agricultural University, Guangzhou, China.
Huangpu Customs Technology Center, China; Guangdong Provincial Key Laboratory for Port Security Intelligent Testing, Guangzhou, China.
J Hazard Mater. 2024 Jan 5;461:132594. doi: 10.1016/j.jhazmat.2023.132594. Epub 2023 Sep 21.
The heightened concern about the environmental impacts of pollutants drives interest in reducing their threats to humans and the environment. Bioremediating polluted sites under environmental stresses like biotic and abiotic poses significant challenges. This study aimed to isolate a bacterium that effectively degrades pyrethroids even under abiotic stresses involving heavy metals and biotic stresses with autochthonous factors. Here, a bacterial strain, Bacillus cereus BCS1 was isolated. The response surface methodology was established to quantify the environmental impacts on pyrethroid degradation. BCS1 effectively degraded pyrethroids across conditions at 21-36 °C, pH 6.5-8.0 and inoculum sizes 1.9-4.1 mg·L, exceeding 90% degradation. Notably, over 84% of β-cypermethrin (β-CP) was degraded even when exposed to various concentrations of lead (10-1000 mg·L), chromium (10-1000 mg·L), or cadmium (0.5-50 mg·L). Moreover, BCS1 significantly accelerated β-CP degradation in soil-plant systems, displaying biotic stress tolerance, with lower half-life values (10.1 and 9.5 d) in soil and higher removal (92.1% and 60.9%) in plants compared to controls (27.7 and 25.7 d), and (18.2% and 24.3%). This study presents a novel strain capable of efficiently degrading pyrethroids and displaying remarkable environmental stress resistance. Findings shed light on bioremediating organic pollutants in complex soil ecosystems.
人们对污染物环境影响的担忧日益加剧，这促使人们致力于减少其对人类和环境的威胁。在生物和非生物等环境压力下，对受污染场地进行生物修复仍然面临着巨大的挑战。本研究旨在分离一种能够在涉及重金属的非生物压力和具有土著因素的生物压力等环境压力下有效降解拟除虫菊酯的细菌。在这里，分离到了一株细菌，即蜡状芽孢杆菌 BCS1。采用响应面法量化了环境因素对拟除虫菊酯降解的影响。BCS1 在 21-36°C、pH6.5-8.0 和接种量为 1.9-4.1mg·L 的条件下能够有效降解拟除虫菊酯，降解率超过 90%。值得注意的是，即使在各种浓度的铅（10-1000mg·L）、铬（10-1000mg·L）或镉（0.5-50mg·L）存在的情况下，仍有超过 84%的β-氯氰菊酯（β-CP）被降解。此外，BCS1 在土壤-植物系统中显著加速了 β-CP 的降解，表现出对生物压力的耐受性，其在土壤中的半衰期值（10.1 和 9.5d）低于对照（27.7 和 25.7d），在植物中的去除率（92.1%和 60.9%）高于对照（18.2%和 24.3%）。本研究提供了一株能够有效降解拟除虫菊酯且具有显著环境压力抗性的新型菌株。研究结果为在复杂的土壤生态系统中生物修复有机污染物提供了新的思路。
