Masoud Atousa, Mohamadynejad Parisa
Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Gene. 2024 Jan 15;891:147828. doi: 10.1016/j.gene.2023.147828. Epub 2023 Sep 23.
Long non-coding RNAs have been implicated in biological processes, and are dysregulated in types of cancer. Studies have shown that PCAT19 and CKMT2-AS1 lncRNAs promote tumor growth, invasion, and metastasis by regulating signaling pathways and modulating the gene expression. This study investigated the expression levels of lncRNAs PCAT19 and CKMT2-AS1 in colorectal tumors and normal tissues. First, Using GEPIA2 database, we compared the expression level of target lncRNAs between primary colon adenocarcinoma tumor and normal tissues. Then, the expression levels of lncRNAs PCAT19 and CKMT2-AS1 were detected in 35 colorectal tumors and paired adjacent tissues using qRT-PCR. A receiver operating characteristic (ROC) curve was used to evaluate the value of these lncRNAs as biomarkers. Statistical analysis based on GEPIA2 showed that both lncRNAs PCAT19 and CKMT2-AS1 were significantly decreased in colon adenocarcinoma compared to the normal group (P < 0.001). Experimental analysis showed that the expression level of lncRNA PCAT19 was decreased in colorectal tumors (p < 0.0001) compared to normal tissues. While the expression level of lncRNA CKMT2-AS1 did not change in tumor tissues, it decreased in non-metastatic tumors compared to normal tissues (p = 0.04). The significantly downregulation of lncRNA PCAT19 expression in both metastatic and non-metastatic colorectal tumors compared to normal tissue suggests that PCAT19 may play a role in the carcinogenesis and progression of colorectal cancer and may provide potential therapeutic targets for colorectal cancer. Based on the results of ROC curve analysis, lncRNA PCAT19 may also serves as a novel potential good biomarker in diagnosis colorectal cancer (AUC = 0.94, p < 0.0001) but no significant was found for lncRNA CKMT2-AS1 (p > 0.05).
长链非编码RNA参与了生物过程，且在多种癌症中表达失调。研究表明，lncRNA PCAT19和CKMT2-AS1通过调节信号通路和调控基因表达促进肿瘤生长、侵袭和转移。本研究调查了lncRNA PCAT19和CKMT2-AS1在结直肠肿瘤组织和正常组织中的表达水平。首先，使用GEPIA2数据库比较原发性结肠腺癌肿瘤组织和正常组织中目标lncRNA的表达水平。然后，采用qRT-PCR检测35例结直肠肿瘤组织及其配对的癌旁组织中lncRNA PCAT19和CKMT2-AS1的表达水平。利用受试者工作特征（ROC）曲线评估这些lncRNA作为生物标志物的价值。基于GEPIA2的统计分析表明，与正常组相比，lncRNA PCAT19和CKMT2-AS1在结肠腺癌中均显著降低（P < 0.001）。实验分析表明，与正常组织相比，lncRNA PCAT19在结直肠肿瘤组织中的表达水平降低（p < 0.0001）。虽然lncRNA CKMT2-AS1在肿瘤组织中的表达水平没有变化，但与正常组织相比，其在非转移肿瘤组织中的表达降低（p = 0.04）。与正常组织相比，lncRNA PCAT19在转移性和非转移性结直肠肿瘤组织中的表达均显著下调，提示PCAT19可能在结直肠癌的发生和发展中发挥作用，并可能为结直肠癌提供潜在的治疗靶点。基于ROC曲线分析结果，lncRNA PCAT19也可能作为一种新型潜在的良好生物标志物用于结直肠癌的诊断（AUC = 0.94，p < 0.0001），但lncRNA CKMT2-AS1未发现显著意义（p > 0.05）。
