Department of Anesthesiology, University of Illinois, Chicago, IL 60612, United States.
Department of Anesthesiology, University of Illinois, Chicago, IL 60612, United States; Department of Veterans Affairs, Jesse Brown VA Medical Center, Chicago, IL 60612, United States.
Neurosci Lett. 2023 Oct 15;815:137497. doi: 10.1016/j.neulet.2023.137497. Epub 2023 Sep 23.
Roles for lipocalin-2 (LCN2, also referred to as neutrophil gelatinase associated lipocalin, NGAL) in the progression of disease in multiple sclerosis and its animal models have been reported; however, the importance of astrocyte-derived LCN2, a major source of LCN2, have not been defined. We found that clinical scores in experimental autoimmune encephalomyelitis (EAE) were modestly delayed in mice with conditional knockout of LCN2 from astrocytes, associated with a small decrease in astrocyte GFAP expression. Immunostaining and qPCR of spinal cord samples showed decreased oligodendrocyte proteolipid protein and transcription factor Olig2 expression, but no changes in PDGFRα expression. These results suggest astrocyte LCN2 contributes to early events in EAE and reduces damage to mature oligodendrocytes at later times.
已有报道称，脂联素-2（LCN2，也称为中性粒细胞明胶酶相关脂质运载蛋白，NGAL）在多发性硬化症及其动物模型中的疾病进展中发挥作用；然而，星形胶质细胞衍生的 LCN2 的重要性（LCN2 的主要来源）尚未确定。我们发现，条件性敲除星形胶质细胞 LCN2 的小鼠的实验性自身免疫性脑脊髓炎（EAE）临床评分略有延迟，与星形胶质细胞 GFAP 表达略有下降相关。脊髓样本的免疫染色和 qPCR 显示少突胶质细胞蛋白脂质蛋白和转录因子 Olig2 的表达减少，但 PDGFRα 表达没有变化。这些结果表明，星形胶质细胞 LCN2 有助于 EAE 的早期事件，并减少后期成熟少突胶质细胞的损伤。
