Zhao Ping, Peng Cheng, Chang Xin, Cheng Wei, Yang Yanhong, Shen Yu, Sun Chao, Feng Xiuyuan, Liu Cuiping, Wu Jian
Department of Rheumatology, The First Affiliated Hospital of Soochow University, No. 188 Shizi St, Suzhou, 215006, Jiangsu, China.
Department of Rheumatology and Clinical Immunology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China.
Clin Rheumatol. 2024 Jan;43(1):297-306. doi: 10.1007/s10067-023-06759-6. Epub 2023 Sep 25.
The purpose of this study was to investigate the expression of T-cell immunoglobulin and ITIM domain (TIGIT) in peripheral circulation of primary Sjögren's syndrome (pSS) and its role in the development of pSS.
The expression of TIGIT on T cells, B cells, natural killer (NK) cells, and CD14 + monocytes was detected by flow cytometry in pSS and healthy control (HC). The correlations between expression of TIGIT and clinical features and laboratory parameters of pSS were analyzed. Meanwhile, we analyzed the change in expression of TIGIT before and after treatment, and its role in the prognosis of pSS treatment was evaluated.
The expression of TIGIT on CD3 + , CD4 + , and CD8 + T cells increased and decreased on CD14 + monocytes in pSS compared to HC; however, there was no significance of B lymphocytes and NK cells. The correlation analysis between the expression of TIGIT on T lymphocytes and CD14 + monocytes and clinical features of pSS showed that the decrease in TIGIT expression on CD14 + monocytes was more closely related to pSS. The expression of TIGIT + CD14 + monocytes negatively correlated with the disease activity of pSS. The expression of TIGIT + CD14 + monocytes of pSS with arthralgia, fatigue, decayed tooth, xerostomia, interstitial lung disease, anti-Ro52 positive, and high IgG decreased compared to that in negative patients. Furthermore, it was significantly lower in active patients than in nonactive patients. After treatment, the expression of TIGIT + CD14 + monocytes tended to increase.
Our study suggested that decreased TIGIT expression on CD14 + monocytes was associated with the clinical manifestations, disease activity, and prognosis of pSS patients. TIGIT + CD14 + monocytes may present as a potential target and a biomarker of the prognosis for immunomodulatory therapy in pSS. Key Points • The expression of TIGIT+CD14+ monocytes significantly decreased in pSS patients compared to HC. • There was a negative correlation between TIGIT+CD14+ monocytes and the disease activity of pSS. • TIGIT+CD14+ monocyte expression was associated with the clinical manifestations, autoantibodies, IgG, and prognosis of pSS patients.
本研究旨在探讨T细胞免疫球蛋白和免疫受体酪氨酸抑制基序结构域（TIGIT）在原发性干燥综合征（pSS）外周血中的表达情况及其在pSS发病机制中的作用。
采用流式细胞术检测pSS患者及健康对照（HC）中T细胞、B细胞、自然杀伤（NK）细胞和CD14⁺单核细胞上TIGIT的表达。分析TIGIT表达与pSS临床特征及实验室指标之间的相关性。同时，分析治疗前后TIGIT表达的变化，并评估其在pSS治疗预后中的作用。
与HC相比，pSS患者CD3⁺、CD4⁺和CD8⁺T细胞上TIGIT的表达增加，而CD14⁺单核细胞上TIGIT的表达降低；然而，B淋巴细胞和NK细胞上TIGIT的表达无显著差异。T淋巴细胞和CD14⁺单核细胞上TIGIT表达与pSS临床特征的相关性分析表明，CD14⁺单核细胞上TIGIT表达的降低与pSS的关系更为密切。TIGIT⁺CD14⁺单核细胞的表达与pSS的疾病活动度呈负相关。与无关节痛、疲劳、龋齿、口干、间质性肺病、抗Ro52阳性及高IgG的pSS患者相比，有关节痛、疲劳、龋齿、口干、间质性肺病、抗Ro52阳性及高IgG的pSS患者TIGIT⁺CD14⁺单核细胞的表达降低。此外，活动期患者的TIGIT⁺CD14⁺单核细胞表达显著低于非活动期患者。治疗后，TIGIT⁺CD14⁺单核细胞的表达呈上升趋势。
我们的研究表明，CD14⁺单核细胞上TIGIT表达降低与pSS患者的临床表现、疾病活动度及预后相关。TIGIT⁺CD14⁺单核细胞可能是pSS免疫调节治疗潜在的靶点和预后生物标志物。要点：•与HC相比，pSS患者TIGIT⁺CD14⁺单核细胞的表达显著降低。•TIGIT⁺CD14⁺单核细胞与pSS的疾病活动度呈负相关。•TIGIT⁺CD14⁺单核细胞的表达与pSS患者的临床表现、自身抗体、IgG及预后相关。
