Tumor Etiology and Screening Department of Cancer Institute and General Surgery, The First Hospital of China Medical University, Shenyang, China.
Department of Radiology, The First Hospital of China Medical University, Shenyang, China.
Ann Med. 2023;55(2):2260395. doi: 10.1080/07853890.2023.2260395. Epub 2023 Sep 26.
Although abdominal aortic aneurysm (AAA) is associated with life-threatening complications, there are still limited reliable biomarkers for diagnostic purpose. MicroRNAs (miRNAs) have been proposed as the potential diagnostic and risk stratification markers of AAA patients, and we aim to evaluate the serum level of miR-1-3p and its diagnostic value in AAA.
This study included 200 AAA patients and 200 controls. Demographic data and clinical information were collected from the subjects' medical records. Individual image analyses of AAA morphology were determined based on computed tomography angiography (CTA). The levels of serum miRNA expression were detected by quantitative real-time PCR. Bioinformatics tools were used to identify the target genes of miR-1-3p and their potential biological functions were further enriched.
Serum miR-1-3p levels in the AAA group were significantly lower when compared with those in the control group in overall and subgroup comparisons. It was negatively related to WBC, CRP, maximal aneurysm diameter, area, and volume in AAA patients. Circulating miR-1-3p levels could significantly discriminate between AAA patients and healthy individuals with an area under the curve (AUC) of 0.672 (95% CI = 0.619-0.724, < 0.001), a sensitivity of 84.5% and a specificity of 45.5%. Serum miR-1-3p was associated with a reduced risk of AAA even after adjustment for possible risk factors (OR = 0.440 per unit increase, 95% CI = 0.301-0.643, < 0.001). And low levels of serum miR-1-3p could significantly elevate the risk of AAA in both univariate and multivariate logistic regression analyses with ORs of 4.076 and 4.136, respectively (all < 0.001). Further GO enrichment analysis revealed that miR-1-3p was mainly involved in negative regulation of apoptotic process, sprouting angiogenesis, angiogenesis, positive regulation of blood vessel endothelial cell migration, positive regulation of cell proliferation, regulation of cell shape, etc.
MiR-1-3p can be used as a promising circulating biomarker in the development of AAA, and it may participate in multiple biological processes to play a crucial role in AAA pathogenesis.
尽管腹主动脉瘤（AAA）与危及生命的并发症有关，但目前仍缺乏可靠的诊断生物标志物。微小 RNA（miRNA）已被提出作为 AAA 患者的潜在诊断和风险分层标志物，本研究旨在评估血清 miR-1-3p 水平及其在 AAA 中的诊断价值。
本研究纳入 200 例 AAA 患者和 200 例对照。从受试者的病历中收集人口统计学数据和临床信息。基于计算机断层血管造影（CTA）进行个体 AAA 形态学图像分析。通过定量实时 PCR 检测血清 miRNA 表达水平。使用生物信息学工具鉴定 miR-1-3p 的靶基因，并进一步富集其潜在的生物学功能。
与对照组相比，AAA 组的血清 miR-1-3p 水平在总体和亚组比较中均显著降低。它与 AAA 患者的白细胞计数、C 反应蛋白、最大瘤径、面积和体积呈负相关。循环 miR-1-3p 水平可显著区分 AAA 患者和健康个体，曲线下面积（AUC）为 0.672（95%CI=0.619-0.724， < 0.001），灵敏度为 84.5%，特异性为 45.5%。即使在调整可能的危险因素后，血清 miR-1-3p 仍与 AAA 的发生风险降低相关（每单位增加 OR=0.440，95%CI=0.301-0.643， < 0.001）。在单因素和多因素 logistic 回归分析中，低水平的血清 miR-1-3p 可显著增加 AAA 的发生风险，OR 分别为 4.076 和 4.136（均 < 0.001）。进一步的 GO 富集分析表明，miR-1-3p 主要参与凋亡过程的负调控、芽生血管生成、血管生成、血管内皮细胞迁移的正调控、细胞增殖的正调控、细胞形状的调节等。
miR-1-3p 可作为 AAA 发生发展中一种有前途的循环生物标志物，可能参与多个生物学过程，在 AAA 发病机制中发挥关键作用。
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