Legaki Evangelia, Klonaris Christos, Athanasiadis Dimitrios, Patelis Nikolaos, Sioziou Anna, Liakakos Theodoros, Gazouli Maria
Department of Basic Medical Sciences, Laboratory of Biology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
First Department of Surgery, Vascular Unit, National and Kapodistrian University of Athens, Laiko General Hospital, Athens, Greece.
In Vivo. 2019 May-Jun;33(3):737-742. doi: 10.21873/invivo.11533.
BACKGROUND/AIM: Nine genetic loci have been associated with abdominal aortic aneurysm (AAA) susceptibility, including DAB2IP. This gene is playing a role in apoptosis, cell proliferation and epithelial-to-mesenchymal transition in cancers. This study aimed to elucidate the differential expression levels of DAB2IP in AAA tissues and investigate whether mir-363-3p and EZH2 can be considered as potential mediators of its expression.
18 AAA samples and 15 non-aneurysmatic controls were collected. Relative mRNA expression levels of DAB2IP, EZH2 and mir-363-3p were measured using qPCR.
DAB2IP was significant up-regulated (~2.29 fold) in AAA tissues, while EZH2 and mir-363-3p were down-regulated (3.28 and 3.62-fold, respectively). A limited negative correlation was found between the DAB2IP and EZH2 expression and between DAB2IP and the mir-363-3p.
An increased expression of DAB2IP in AAA tissues was shown. We suggest 2 potential mediators of DAB2IP expression in abdominal aortic aneurysm, EZH2 and mir-363-3p.
背景/目的：包括DAB2IP在内的9个基因位点与腹主动脉瘤（AAA）易感性相关。该基因在癌症的细胞凋亡、细胞增殖和上皮-间质转化中发挥作用。本研究旨在阐明DAB2IP在AAA组织中的差异表达水平，并研究mir-363-3p和EZH2是否可被视为其表达的潜在调节因子。
收集18例AAA样本和15例非动脉瘤对照。使用qPCR测量DAB2IP、EZH2和mir-363-3p的相对mRNA表达水平。
DAB2IP在AAA组织中显著上调（约2.29倍），而EZH2和mir-363-3p下调（分别为3.28倍和3.62倍）。DAB2IP与EZH2的表达之间以及DAB2IP与mir-363-3p之间存在有限的负相关。
结果显示AAA组织中DAB2IP表达增加。我们提出EZH2和mir-363-3p是腹主动脉瘤中DAB2IP表达的2个潜在调节因子。
