用无人机进行器官运输的可行性：一项使用大鼠模型的实验研究。

Feasibility of Organ Transportation by a Drone: An Experimental Study Using a Rat Model.

机构信息

Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.

出版信息

Transplant Proc. 2023 Nov;55(9):2227-2231. doi: 10.1016/j.transproceed.2023.08.018. Epub 2023 Sep 24.

DOI:10.1016/j.transproceed.2023.08.018

PMID:37752014

Abstract

BACKGROUND

Recently, the successful delivery of organs for transplantation using drones was reported. We investigated the influence of transportation by drones on the quality of liver grafts using a rat model.

METHODS

Livers of 12 rats (8 and 32 weeks old) were divided into 2 groups of six. Livers were split into 2 parts and allocated to the drone or control groups (both n = 12). The drone experiment was conducted between islands in Nagasaki Prefecture, Japan. The distance between the islands was 12 km. Livers of the drone group were transported by a multicopter at a speed of 30 km-40 km/h over 60 m above sea level. Transported liver quality was analyzed by histology, and biochemistry data were compared between groups.

RESULTS

Cold ischemia time did not differ between groups (902 min and 909 min, respectively). There were no differences in macroscopic findings regarding coloration and damage between groups. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) in preservation fluid were graft weight-corrected and compared, and no significant differences were found between groups: AST/g (4.61 vs 4.81 IU/L), ALT/g (2.78 vs 2.92 IU/L), and ALP/g (39.1 vs 37.0 IU/L). Immunochemical staining showed no significant difference between groups for terminal deoxynucleotidyl transferase dUTP nick and labeling staining (141 vs 113 cells), CD163 (818 vs 870 cells), and TNF-α (1.25 vs 1.41 scores).

CONCLUSIONS

The simulation experiment of organ transport for transplantation by drones was successfully conducted. There were no differences in the quality of livers transported by drones or other means. Further studies including large-animal experiments could lead to future clinical applications.

摘要

背景

最近，有报道称使用无人机成功运送器官进行移植。我们使用大鼠模型研究了使用无人机运输对肝移植物质量的影响。

方法

将 12 只大鼠（8 周龄和 32 周龄）的肝脏分为两组，每组 6 只。将肝脏分为两部分，并分配到无人机组或对照组（每组 n=12）。无人机实验在日本长崎县的两个岛屿之间进行。两个岛屿之间的距离为 12 公里。无人机组的肝脏通过多旋翼飞机以 30-40 公里/小时的速度在海拔 60 米以上的高度运输。通过组织学分析运输后的肝脏质量，并比较组间的生化数据。

结果

两组的冷缺血时间无差异（分别为 902 分钟和 909 分钟）。两组间在肝脏颜色和损伤的宏观表现方面无差异。保存液中天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）和碱性磷酸酶（ALP）与供体体重校正后比较，两组间无显著差异：AST/g（4.61 比 4.81 IU/L）、ALT/g（2.78 比 2.92 IU/L）和 ALP/g（39.1 比 37.0 IU/L）。免疫化学染色显示，两组间末端脱氧核苷酸转移酶 dUTP 缺口末端标记染色（141 比 113 个细胞）、CD163（818 比 870 个细胞）和 TNF-α（1.25 比 1.41 分）均无显著差异。