Salt toxicity is one of the foremost environmental stresses that declines nutrient uptake, photosynthetic activity and growth of plants resulting in a decrease in crop yield and quality. Seed priming has become an emergent strategy to alleviate abiotic stress and improve plant growth. During the current study, turnip seed priming with sodium selenite (NaSeO) was investigated for its ability to mitigate salt stress. Turnip ( L. var. Purple Top White Globe) seeds primed with 75, 100, and 125 ML of Se were subjected to 200 mM salt stress under field conditions. Findings of the current field research demonstrated that salt toxicity declined seed germination, chlorophyll content, and gas exchange characteristics of seedling. Whereas, Se-primed seeds showed higher germination rate and plant growth which may be attributed to the decreased level of hydrogen peroxide (HO) and malondialdehyde (MDA) decreased synthesis of proline (36%) and besides increased total chlorophyll (46%) in applied turnip plants. Higher expression levels of genes encoding antioxidative activities (, , and ) mitigated oxidative stress induced by the salt toxicity. Additionally, Se treatment decreased Na content and enhanced K content resulting in elevated K/Na ratio in the treated plants. The assessment revealed the interactive superiority of Se with antioxidant enzymes including CAT, POD, SOD, and APX as compared to sodium chloride (NaCl). Computational study of enzymes-Se and enzymes-NaCl molecules also revealed the stress ameliorative potential of Se through the presence of more Ramachandran-favored regions (94%) and higher docking affinities of Se (-6.3). The studies through molecular docking of NaSeO, NaCl, and ROS synthesizing (receptors) including cytochrome P450 (CYP), (LOX), and xanthine oxidase (XO), also confirmed the salt stress ameliorative potential of Se in . The increased Ca, P, Mg, and Zn nutrients uptake nutrients uptake in 100 ML Se primed seedlings helped to adjust the stomatal conductivity (35%) intercellular CO concentration (32%), and photosynthetic activity (41%) resulting in enhancement of the yield attributes. More number of seeds per plant (6%), increased turnip weight (115 gm) root length (17.24 cm), root diameter (12 cm) as well as turnip yield increased by (9%tons ha) were recorded for 100 ML Se treatment under salinity stress. Findings of the current research judiciously advocate the potential of Se seed priming for salt stress alleviation and growth improvement in .