Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran 1458889694, Iran.
Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran 1458889694, Iran.
Biosensors (Basel). 2023 Aug 23;13(9):837. doi: 10.3390/bios13090837.
Lateral flow tests are one of the most important types of paper-based point-of-care (POCT) diagnostic tools. It shows great potential as an implement for improving the rapid screening and management of infections in global pandemics or other potential health disorders by using minimally expert staff in locations where no sophisticated laboratory services are accessible. They can detect different types of biomarkers in various biological samples and provide the results in a little time at a low price. An important challenge regarding conventional LFAs is increasing their sensitivity and specificity. There are two main approaches to increase sensitivity and specificity, including assay improvement and target enrichment. Assay improvement comprises the assay optimization and signal amplification techniques. In this study, a summarize of various sensitivity and specificity enhancement strategies with an objective evaluation are presented, such as detection element immobilization, capillary flow rate adjusting, label evolution, sample extraction and enrichment, etc. and also the key findings in improving the LFA performance and solving their limitations are discussed along with numerous examples.
侧向流检测是最重要的基于纸的即时护理（POCT）诊断工具之一。它具有很大的潜力，可以通过在无法获得复杂实验室服务的地方使用最少的专家人员，改善全球大流行或其他潜在健康障碍感染的快速筛查和管理。它们可以检测各种生物样本中的不同类型的生物标志物，并以低廉的价格在短时间内提供结果。关于传统 LFAs 的一个重要挑战是提高其灵敏度和特异性。提高灵敏度和特异性有两种主要方法，包括检测方法的改进和目标富集。检测方法的改进包括检测方法的优化和信号放大技术。在本研究中，对各种提高灵敏度和特异性的策略进行了总结，并进行了客观评价，例如检测元件固定化、毛细流速调节、标记物演变、样品提取和富集等，同时还讨论了提高 LFAs 性能和解决其局限性的关键发现，并提供了大量的实例。
