Department of Fibre and Polymer Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Teknikringen 56, 10044 Stockholm, Sweden.
Biosensors (Basel). 2023 Aug 25;13(9):844. doi: 10.3390/bios13090844.
Analytical systems based on isothermal nucleic acid amplification tests (NAATs) paired with electroanalytical detection enable cost-effective, sensitive, and specific digital pathogen detection for various in situ applications such as point-of-care medical diagnostics, food safety monitoring, and environmental surveillance. Self-assembled monolayers (SAMs) on gold surfaces are reliable platforms for electroanalytical DNA biosensors. However, the lack of automation and scalability often limits traditional chip-based systems. To address these challenges, we propose a continuous thread-based device that enables multiple electrochemical readings on a functionalized working electrode Au thread with a single connection point. We demonstrate the possibility of rolling the thread on a spool, which enables easy manipulation in a roll-to-roll architecture for high-throughput applications. As a proof of concept, we have demonstrated the detection of recombinase polymerase amplification (RPA) isothermally amplified DNA from the two toxic microalgae species cf. and cf. by performing a sandwich hybridization assay (SHA) with electrochemical readout.
基于等温核酸扩增试验（NAAT）与电化学生物传感器相结合的分析系统，为各种原位应用（如即时医疗诊断、食品安全监测和环境监测）提供了具有成本效益、灵敏和特异的数字病原体检测方法。金表面上的自组装单分子层（SAM）是电化学生物 DNA 传感器的可靠平台。然而，自动化和可扩展性的缺乏常常限制了传统的基于芯片的系统。为了解决这些挑战，我们提出了一种连续线程式设备，该设备能够在带有单个连接点的功能化工作电极 Au 线程上进行多次电化学生物传感器读数。我们展示了将线程卷在卷轴上的可能性，这使得在用于高通量应用的卷对卷架构中易于操作。作为概念验证，我们通过夹心杂交测定（SHA）并进行电化学生物传感器检测，展示了对两种有毒微藻 cf. 和 cf. 的等温扩增 RPA 扩增 DNA 的检测。
