Chen Changdong, La Ming, Yi Xinyao, Huang Mengjie, Xia Ning, Zhou Yanbiao
College of Chemical and Environmental Engineering, Pingdingshan University, Pingdingshan 476000, China.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Biosensors (Basel). 2023 Aug 29;13(9):855. doi: 10.3390/bios13090855.
Electrochemical immunosensors have shown great potential in clinical diagnosis, food safety, environmental protection, and other fields. The feasible and innovative combination of enzyme catalysis and other signal-amplified elements has yielded exciting progress in the development of electrochemical immunosensors. Alkaline phosphatase (ALP) is one of the most popularly used enzyme reporters in bioassays. It has been widely utilized to design electrochemical immunosensors owing to its significant advantages (e.g., high catalytic activity, high turnover number, and excellent substrate specificity). In this work, we summarized the achievements of electrochemical immunosensors with ALP as the signal reporter. We mainly focused on detection principles and signal amplification strategies and briefly discussed the challenges regarding how to further improve the performance of ALP-based immunoassays.
电化学免疫传感器在临床诊断、食品安全、环境保护等领域展现出了巨大潜力。酶催化与其他信号放大元件的可行且创新的结合，在电化学免疫传感器的发展中取得了令人振奋的进展。碱性磷酸酶（ALP）是生物分析中最常用的酶报告分子之一。由于其显著优势（如高催化活性、高周转数和优异的底物特异性），它已被广泛用于设计电化学免疫传感器。在这项工作中，我们总结了以ALP作为信号报告分子的电化学免疫传感器的成果。我们主要关注检测原理和信号放大策略，并简要讨论了在如何进一步提高基于ALP的免疫分析性能方面所面临的挑战。
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