Zhang Ze-Long, Wang Xiao-Jing, Lu Hai-Bin, Huang Hai-Jian
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Insects. 2023 Aug 31;14(9):739. doi: 10.3390/insects14090739.
The bean bug, (Hemiptera: Heteroptera), poses a significant threat to soybean production, resulting in substantial crop losses. Throughout the soybean cultivation period, these insects probe and suck on various parts of plants, including leaves, pods, and beans. However, the specific mechanisms by which they adapt to different food resources remain unknown. In this study, we conducted gut transcriptomic analyses of fed with soybean leaves, pods, and beans. A total of 798, 690, and 548 differently expressed genes (DEGs) were monitored in G-pod vs. G-leaf (comparison of insect feeding on pods and leaves), G-bean vs. G-leaf (comparison of insect feeding on beans and leaves), and G-pod vs. G-bean (comparison of insect feeding on pods and beans), respectively. When fed on pods and beans, there was a significant increase in the expression of digestive enzymes, particularly cathepsins, serine proteases, and lipases. Conversely, when soybean leaves were consumed, detoxification enzymes, such as ABC transporters and 4-coumarate-CoA ligase, exhibited higher expression. Our findings indicate that dynamically regulates different metabolic pathways to cope with varying food resources, which may contribute to the development of effective strategies for managing this pest.
豆蝽（半翅目：异翅亚目）对大豆生产构成重大威胁，导致大量作物损失。在整个大豆种植期，这些昆虫会刺探并吸食植物的各个部位，包括叶片、豆荚和豆子。然而，它们适应不同食物资源的具体机制仍不清楚。在本研究中，我们对取食大豆叶片、豆荚和豆子的豆蝽进行了肠道转录组分析。在G-豆荚与G-叶片（昆虫取食豆荚和叶片的比较）、G-豆子与G-叶片（昆虫取食豆子和叶片的比较）以及G-豆荚与G-豆子（昆虫取食豆荚和豆子的比较）中，分别监测到798、690和548个差异表达基因（DEGs）。当取食豆荚和豆子时，消化酶的表达显著增加，尤其是组织蛋白酶、丝氨酸蛋白酶和脂肪酶。相反，当取食大豆叶片时，解毒酶如ABC转运蛋白和4-香豆酸-CoA连接酶表现出更高的表达。我们的研究结果表明，豆蝽动态调节不同的代谢途径以应对不同的食物资源，这可能有助于制定有效的害虫管理策略。
