Ribeiro Thyago Fernando Lisboa, Oliveira Demetrios José de Albuquerque, da Costa João Gomes, Gutierrez Miguel Angel Martinez, de Oliveira Eder Jorge, Ribeiro Junior Karlos Antonio Lisboa, Goulart Henrique Fonseca, Riffel Alessandro, Santana Antonio Euzebio Goulart
Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió 57072-900, AL, Brazil.
Embrapa Food and Territories, Maceió 57020-050, AL, Brazil.
Insects. 2023 Sep 13;14(9):762. doi: 10.3390/insects14090762.
Cassava is an essential tuber crop used to produce food, feed, and beverages. Whitefly pests, including (Goeldi, 1886) (Hemiptera: Aleyrodidae), significantly affect cassava-based agroecosystems. Plant odours have been described as potential pest management tools, and the cassava clone M Ecuador 72 has been used by breeders as an essential source of resistance. In this study, we analysed and compared the volatile compounds released by this resistant clone and a susceptible genotype, BRS Jari. Constitutive odours were collected from young plants and analysed using gas chromatography-mass spectrometry combined with chemometric tools. The resistant genotype released numerous compounds with previously described biological activity and substantial amounts of the monoterpene ()-β-ocimene. Whiteflies showed non-preferential behaviour when exposed to volatiles from the resistant genotype but not the susceptible genotype. Furthermore, pure ocimene caused non-preferential behaviour in whiteflies, indicating a role for this compound in repellence. This report provides an example of the intraspecific variation in odour emissions from cassava plants alongside information on odorants that repel whiteflies; these data can be used to devise whitefly management strategies. A better understanding of the genetic variability in cassava odour constituents and emissions under field conditions may accelerate the development of more resistant cassava varieties.
木薯是一种重要的块茎作物，用于生产食品、饲料和饮料。包括烟粉虱（戈埃尔迪，1886年）（半翅目：粉虱科）在内的粉虱害虫对以木薯为基础的农业生态系统有显著影响。植物气味已被描述为潜在的害虫管理工具，木薯品种M Ecuador 72已被育种者用作重要的抗性来源。在本研究中，我们分析并比较了这种抗性品种和易感基因型BRS Jari释放的挥发性化合物。从幼苗中收集组成性气味，并使用气相色谱-质谱联用化学计量工具进行分析。抗性基因型释放出许多具有先前描述的生物活性的化合物以及大量的单萜（）-β-罗勒烯。粉虱暴露于抗性基因型的挥发物时表现出非偏好行为，但暴露于易感基因型的挥发物时则不然。此外，纯罗勒烯会使粉虱表现出非偏好行为，表明该化合物在驱避方面发挥作用。本报告提供了木薯植株气味排放种内变异的一个例子，以及关于驱避粉虱的气味物质的信息；这些数据可用于制定粉虱管理策略。更好地了解田间条件下木薯气味成分和排放的遗传变异性，可能会加速培育更具抗性的木薯品种。
