Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, No. 723 Xingke Road, Tianhe District, Guangzhou 510650, China.
University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China.
J Agric Food Chem. 2021 Sep 29;69(38):11204-11215. doi: 10.1021/acs.jafc.1c03534. Epub 2021 Sep 20.
Herbivore-induced plant volatiles (HIPVs) play an important role in insect resistance. As a common HIPV in tea plants (), β-ocimene has shown anti-insect function in other plants. However, whether β-ocimene in tea plants also provides insect resistance, and its mechanism of synthesis and emission are unknown. In this study, β-ocimene was confirmed to interfere with tea geometrid growth signaling. Light was identified as the key factor controlling regular emission of β-ocimene induced by the wounding from tea geometrids. β-Ocimene synthase (CsBOS1) was located in plastids and catalyzed β-ocimene formation in overexpressed tobacco. expression in tea leaves attacked by tea geometrids showed a day-low and night-high variation pattern, while expression involved in volatile emission showed the opposite pattern. These two genes might regulate the regular β-ocimene emission from tea plants induced by tea geometrid attack. This study advances the understanding on HIPV emission and signaling in tea plants.
植物挥发物（HIPVs）在昆虫抗性中起着重要作用。β-罗勒烯（）作为茶树中常见的 HIPV，已在其他植物中表现出抗虫功能。然而，茶树中的β-罗勒烯是否也提供了昆虫抗性，以及其合成和排放的机制尚不清楚。在这项研究中，β-罗勒烯被证实干扰了茶树茶尺蠖的生长信号。光被确定为控制由茶尺蠖刺伤诱导的β-罗勒烯规律排放的关键因素。β-罗勒烯合酶（CsBOS1）位于质体中，并在过量表达的烟草中催化β-罗勒烯的形成。在受到茶尺蠖攻击的茶树叶片中，表达表现出日低夜高的变化模式，而涉及挥发物排放的表达则呈现相反的模式。这两个基因可能调节由茶尺蠖攻击诱导的茶树中规律的β-罗勒烯排放。本研究提高了对茶树 HIPV 排放和信号转导的认识。
