Lukhtanov Vladimir A, Shapoval Nazar A, Dantchenko Alexander V, Eckweiler Wolfgang
Department of Karyosystematics, Zoological Institute, Russian Academy of Sciences, Universitetskaya Nab. 1, 199034 Saint-Petersburg, Russia.
Gronauer Street 40, D-60385 Frankfurt, Germany.
Insects. 2023 Sep 15;14(9):769. doi: 10.3390/insects14090769.
The need for multi-gene analysis in evolutionary and taxonomic studies is generally accepted. However, the sequencing of multiple genes is not always possible. For various reasons, short mitochondrial DNA barcodes are the only source of molecular information for some species in many genera, although multi-locus data are available for other species of the same genera. In particular, such situation exists in the species-rich butterfly subgenus (). Here, we analyzed the partitioning of this subgenus into species groups by using three data sets. The first data set was represented by short mitochondrial DNA barcodes for all analyzed samples. The second and third data sets were represented by a combination of short mitochondrial DNA barcodes for part of the taxa with longer mitochondrial sequences + + (data set 2) and with longer mitochondrial + + and nuclear + + sequences (data set 3) for the remaining species. We showed that the DNA barcoding approach (data set 1) failed to reveal the phylogenetic structure, resulting in numerous polytomies in the tree obtained. Combined analysis of the mitochondrial and nuclear sequences (data sets 2 and 3) revealed the species groups and the position within these species groups, even for the taxa for which only short DNA barcodes were available.
在进化和分类学研究中进行多基因分析的必要性已得到普遍认可。然而，对多个基因进行测序并不总是可行的。由于各种原因，短线粒体DNA条形码是许多属中某些物种唯一的分子信息来源，尽管同一属的其他物种可获得多位点数据。特别是，在物种丰富的蝴蝶亚属（）中存在这种情况。在这里，我们使用三个数据集分析了该亚属划分为物种组的情况。第一个数据集由所有分析样本的短线粒体DNA条形码表示。第二个和第三个数据集由部分分类单元的短线粒体DNA条形码与其余物种的较长线粒体序列++（数据集2）以及较长线粒体++和核++序列（数据集3）的组合表示。我们表明，DNA条形码方法（数据集1）未能揭示系统发育结构，导致所得树中出现大量多歧分支。线粒体和核序列的联合分析（数据集2和3）揭示了物种组以及这些物种组内的位置，即使对于仅具有短DNA条形码的分类单元也是如此。
